荔枝核总黄酮对肝纤维化大鼠核转录因子-κB及基质金属蛋白酶-2表达的影响  

Effect of total flavone from Litchi chinensis Sonn. on the expressions of NF-κB and MMP-2 in rats with hepatic fibrosis

作　　者：罗伟生[1] 欧士钰[2] 靳雅玲[2] 覃浩[2] 孙旭锐[2] 

机构地区：[1]广西中医药大学第一附属医院消化内科,南宁530001 [2]桂林医学院附属医院消化内科,桂林541004

出　　处：《重庆医科大学学报》2012年第11期943-948,共6页Journal of Chongqing Medical University

基　　金：广西卫生厅重点课题资助项目(编号:2010052);广西中医药药效研究重点实验室开放课题资助项目(编号:10-046-04-k12)

摘　　要：目的:观察核转录因子-κB(Nuclear transcription factors-κB,NF-κB)及基质金属蛋白酶-2(Matrix metalloproteinases-2,MMP-2)在实验性大鼠肝纤维化肝组织中的表达,探讨荔枝核总黄酮(Total flavone from Litchi chinensis Sonn.,TFL)抗肝纤维化的作用机制。方法:大鼠随机分为正常对照组、模型组、TFL高剂量组、TFL低剂量组及秋水仙碱(Colchicine,Col)组。模型组、TFL高剂量组、TFL低剂量组及Col组以二甲基亚硝胺(Dimethylnitrosamine,DMN)腹腔注射4周制作大鼠肝纤维化模型;造模同时TFL高、低剂量组分别以TFL[200、100 mg/(kg.d)],Col组以Col 0.1 mg/(kg.d)灌胃给药,正常对照组及模型组给予等体积生理盐水灌胃,6周后处死大鼠,计算大鼠肝脾指数,检测血清天冬氨酸氨基转移酶(Aspartate aminotransferase,AST)、丙氨酸氨基转移酶(Alanine aminotransferase,ALT)、透明质酸(Hyaluronic acid,HA)、层粘连蛋白(Laminin,LN)、MMP-2,取肝脏同一部位行HE、Masson染色观察大鼠肝纤维化程度,采用免疫组化检测各组肝组织NF-κB、MMP-2的表达。结果:TFL高、低剂量组血清AST、ALT、HA、LN、MMP-2水平及肝脾指数较模型组明显降低(P<0.01),肝组织NF-κB、MMP-2表达及纤维化程度评分亦较模型组明显减低(P<0.05)。结论:TFL具有显著的抗肝纤维化作用,其机制可能与其抑制NF-κB、MMP-2的高表达有关。Objective：To observe the levels of nuclear transcription factors-κB（NF-κB）and matrix metalloproteinases-2（MMP-2） in the liver tissues of rats with hepatic fibrosis and to explore the anti-hepatic fibrosis mechanism of total flavone from Litchi chinensis Sonn.（TFL）.Methods ：Rats were randomized into normal control group,model group,TFL high dose group,TFL low dose group and colchicine group.Rat model of hepatic fibrosis was established by intraperitoneal injection of dimethylnitrosamine for 4 weeks.At the same time,TFL was orally given to fibrosis rats at a dose of 100 or 200 mg/（kg.d）,colchicine was administrated intragastrically at a dose of 0.1 mg/（kg.d）and equal amount of normal saline was given to rats in normal control group and model group.After 6 weeks,all rats were sacrificed and their left liver lobes were examined histopathologically.The liver and spleen index was figured out.The degrees of fibrosis were evaluated by HE and Masson straining.Serum trail levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,hyaluronic acid（HA）,laminin（LN）and MMP-2 were detected.Expressions of NF-κB and MMP-2 in liver tissues were assessed by SP immunohistochemical staining.Results：The liver and spleen index and the serum trail levels of ALT,AST,HA,LN and MMP-2 were significantly lower in TFL high dose and low dose groups than in model group（P〈0.01）.Degrees of hepatic fibrosis were less severe in TFL high dose and low dose groups than in model group（P〈0.05）.Expression levels of NF-κB and MMP-2 were lower in TFL high dose and low dose groups than in model group（P〈0.05）.Conclusions：TEL has obvious anti-hepatic fibrosis effect;the mechanism possibly correlates with the inhibition of NF-κB and MMP-2 expression.

关 键 词：荔枝核总黄酮 肝纤维化 核转录因子-ΚB 基质金属蛋白酶-2 

分 类 号：R575.2[医药卫生—消化系统]