克瘤丸对结肠癌细胞HT-29细胞凋亡及细胞周期的影响  

Effect of Keliu Pill on the Apoptosis and Cell Cycle of Human Colon Carcinoma Cell Line HT-29

作　　者：王瑞平[1] 邹玺[1] 黄舒然[1] 

机构地区：[1]江苏省中医院肿瘤科

出　　处：《中医杂志》2012年第21期1852-1855,共4页Journal of Traditional Chinese Medicine

基　　金：江苏省六大人才高峰资助项目(2008023);江苏省中医药领军人才资助项目(LJ200908)

摘　　要：目的观察克瘤丸对人结肠癌细胞生长的影响及其作用机制。方法将4mg/ml、2mg/ml、1mg/ml、0.5mg/ml、0.25mg/ml浓度克瘤丸作用于HT-29细胞24～48h后,用四甲基偶氮唑蓝(MTT)法检测克瘤丸对HT-29细胞增殖的影响,AnnexinⅤ单染法检测克瘤丸对HT-29细胞凋亡的影响,流式细胞仪检测细胞周期的分布情况。结果与对照组比较,浓度为4、2、1、0.5mg/ml克瘤丸作用HT-29细胞48h,OD值、48h抑制率差异均有统计学意义(P<0.01);4、2、1mg/ml浓度克瘤丸作用HT-29细胞48h,OD值、48h抑制率均明显优于0.25mg/ml及0.5mg/ml浓度克瘤丸(P<0.01)。与1mg/ml浓度比较,2mg/ml、4mg/ml组凋亡率差异有统计学意义(P<0.05)。4mg/ml时G0/G1期前出现明显的亚二倍体区,与1mg/ml、2mg/ml组比较,差异有统计学意义(P<0.05)。结论克瘤丸对人结肠癌细胞HT-29的增殖具有抑制作用并能够引起该细胞的凋亡。Objective To observe the effect and mechanism of Keliu Pill （antitumor pill） on the growth of human colon carcinoma cell. Methods The cell proliferation was assessed by methyl-thiazolyl-tetrazolium （MTT） assay after 24 to 48 hours of treatment with different concentrations （4, 2, 1, 0.5 or 0.25mg/ml） of Keliu Pill on HT-29 cells. The apoptosis was determined with Annexin V staining and cell cycleand was determined with flow cytometry （FCM）. Results Comparing with the control group, Keliu Pill at different concentrations （0.5 to 4 mg/ml） could significantly inhibit the proliferation of HT-29 cells, with statistical significance in the 48h cellular proliferation inhibition rate （CPIR） and optical density （OD） value （P〈 0.01）. The OD values and 48h CPIRs of Keliu Pill at high concentrations （lmg/ml to 4mg/ml） were superior to those at low concentrations （0.25mg/ml and 0.5mg/ml）, with statistical significance （P〈0.01）. Comparing with the group of lmg/ml, the apoptosis rates were significantly increased in group of 2 and 4mg/ml of Keliu Pill （P〈0. 05）. The apparent hypodiploid area appeared before G0/G1 phase after treatment with 4mg/ml of Keliu Pill, with significant difference as compared with ling/ ml and 2mg/ml of Keliu Pill （P〈0.05）. Conclusion Keliu Pill could inhibit the proliferation and induce the apoptosis of human colon carcinoma cell line HT-29.

关 键 词：克瘤丸 结肠癌细胞HT-29 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R735.35[医药卫生—肿瘤]