星形胶质细胞条件培养液对缺氧损伤神经元的影响  

Influence of astrocyte-conditioned medium on anoxia damaged neurons

作　　者：徐建可[1] 栗延伟[2] 韩新生 谭军[2] 

机构地区：[1]新乡医学院第三临床学院,河南新乡453003 [2]新乡医学院第三附属医院神经内科,河南新乡453003 [3]开封市第一人民医院神经内科,河南开封475000

出　　处：《新乡医学院学报》2012年第10期750-753,共4页Journal of Xinxiang Medical University

摘　　要：目的分析星形胶质细胞(Ast)与腺苷对缺氧/复氧损伤神经元的保护作用,揭示腺苷对神经系统的保护机制。方法体外培养SD大鼠大脑皮层Ast和海马神经元及大脑皮层神经元,纯化后给予神经元缺氧/复氧处理,收集复氧18 h后的Ast条件培养液(ACM)和腺苷预处理的Ast条件培养液(ACMa),然后用ACM、ACMa及ACM+腺苷(ACM+含100μmol·L-1腺苷的DMEM液)以15的浓度培养缺氧损伤神经元;光镜观察神经元形态学变化,二甲氧唑黄比色法测定细胞活性。结果 ACMa组、ACM+腺苷组及ACM组的神经元缺氧损伤后的细胞形态较模型组得到明显改善,细胞活性较模型组也得到显著提高。不同条件培养液对缺氧/复氧后神经元活性的作用:ACMa>ACM+腺苷>ACM。结论 Ast条件培养液对缺氧/复氧损伤的神经元有重要的保护、修复作用,腺苷可通过Ast间接地保护和修复受损神经元。Objective To analyse the protective effect of astrocytes and adenosine on the hypoxia / reoxygenation damaged neurons for further revealing the neuro-protective mechanisms of adenosine.Methods SD rat cerebral cortical astrocytes and astrocytes,hippocampal neurons were cultured in virto,neurons and hippocampal neurons were purified to establish models of hypoxia/reoxygenation injury.After reoxygenations for 18 hours,astrocyte condition medium（ACM） and adenosine-preconditioning astrocyte condition medium（ACMa） were collected.Then the damaged neurons,from models of hypoxia/reoxygenation injury were cultured in ACM,ACMa and ACM＋adenosine（ACM mixed with DMEM medium containing 100 μmol·L-1 adenosine）.The concentration was 15.Light microscopy was used to observe the morphological changes of neurons and the neuronal activity was detected by XTT.Results Compared with model group,the cell morphology and neuronal activity improved obviously after neurons induced by hypoxia injury in ACMa group,ACM＋ adenosine group and ACM group.Their roles to the neuronal activity presumed to be：ACMa ACM＋adenosine ACM.Conclusion Astrocyte-conditioned medium plays an important role in protecting and restoring damaged neurons.Adenosine can indirectly protect and repair damaged nerves through astrocytes,which plays key roles in hypoxia/reoxygenation injury.

关 键 词：腺苷 缺氧 复氧 神经元 星形胶质细胞条件培养液 

分 类 号：R741.05[医药卫生—神经病学与精神病学]