唐菖蒲脂氧合酶基因GhLOX1对球茎膨大的影响  

Effects of GhLOX1 Gene on the Corm Enlargement in Gladiolus hybridus

作　　者：连青龙[1,2] 辛海波[2,3] 李晓昕[2] 钟雄辉[2] 尹义蕾[1] 义鸣放[2] 

机构地区：[1]农业部规划设计研究院设施农业研究所,北京100125 [2]中国农业大学观赏园艺与园林系,北京100193 [3]中国科学院植物分子生理学重点实验室,北京100093

出　　处：《园艺学报》2012年第10期1983-1990,共8页Acta Horticulturae Sinica

基　　金：公益性行业(农业)科研专项(200903020;200903009)

摘　　要：以唐菖蒲(Gladiolus hybridus)品种'Rose Supreme'的球茎为试材,应用Real time RT-PCR技术,分析唐菖蒲球茎在不同浓度的茉莉酸甲酯(methyl jasmonate,MJ)0.1、0.2、0.5mmol·L-1和水杨酸异羟肟酸(salicylhydroxamic acid,SHAM)0.5、0.75、1.0mmol·L-1处理后脂氧合酶(LOX)基因的的表达水平,并且对LOX活性、内源MJ含量以及球茎鲜样质量与体积进行测定。分析结果表明,GhLOX1表达水平、脂氧合酶活性、内源MJ含量以及球茎的鲜样质量与体积均随着MJ浓度的增加而逐渐提高。与此相反,经过不同浓度的LOX抑制剂SHAM处理后,以上各项指标随着SHAM浓度的增加而逐渐降低。同时,构建35S-GhLOX1过表达载体,利用根癌农杆菌介导法将GhLOX1导入马铃薯栽培品种'中薯3号'的试管薯薄片中,转基因马铃薯植株块茎的数量、体积和鲜样质量分别得到了不同程度的提高。The expression level of GhLOX1 gene was analyzed in the corm of Gladiolus hybridus ‘Rose Supreme’by Real time RT-PCR,which were treated by different concentration of MJ（0.1,0.2, 0.5 mmol·L-1 ）and SHAM（0.5,0.75,1.0 mmol·L-1）respectively. In addition,LOX activity, endogenesis MJ content,fresh weight and size of corms were also assayed. Analysis results showed that the expression level of GhLOX1 gene,LOX activity and endogenous MJ content,fresh weight and size of corms increased steadily with a MJ gradient from 0.1 mmol·L-1 to 0.5 mmol·L-1 . Conversely,these indexes steadily decreased with the increasing concentration of SHAM. At the same time,35S-GhLOX1 over expression vector was constructed,and GhLOX1 gene was transformed into the microtuber slices of potato cultivar‘Zhongshu 3’by Agrobacterium tumefaciens-mediated method. The results showed that the number,size and fresh weight of transgenic potato tubers had been increased to varying degrees.

关 键 词：唐菖蒲 脂氧合酶 茉莉酸甲酯 水杨酸异羟肟酸 球茎膨大 

分 类 号：S682.24[农业科学—观赏园艺]