环六缩酚肽抑制人前列腺癌细胞增殖活性及作用机制研究  

Inhibotory effect of cyclic hexadepsipeptides on the protiferation activity of human PC-3 cells

作　　者：倪晓辰[1] 张爱莉[1] 赵志红[1] 史清文[2] 

机构地区：[1]河北医科大学第四医院泌尿外科,河北石家庄050011 [2]河北医科大学药学院,河北石家庄050017

出　　处：《中国药理学通报》2012年第11期1527-1530,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81072551);河北省自然科学基金资助项目(No C2010000489);河北省科技支撑项目(No 11276103D-89)

摘　　要：目的研究从微生物次生代谢产物中纯化的3种环六缩酚肽对人前列腺癌细胞(PC-3)增殖的抑制作用,并进一步探讨其可能的作用机制。方法 MTT比色法检测细胞增殖抑制率;TUNEL技术检测PC-3细胞凋亡;Western印迹技术检测Bax和caspase-3蛋白的表达变化。结果 MTT法实验结果显示10μmol·L-1浓度的Pullularin C对PC-3细胞增殖有明显抑制作用,与对照组相比差异有显著性(P<0.01);TUNEL检测结果显示1μmol·L-1浓度的PullularinC处理组的细胞凋亡率明显高于阴性对照组(P<0.01);Western blot分析结果显示由Pullularin C处理的PC-3细胞中的Bax和caspase-3的表达与对照组相比有明显的增加(P<0.05)。结论 Pullularin C具有极强的抑制人前列腺癌细胞增殖作用,并可诱导其凋亡。Pullularin C诱导PC-3细胞凋亡可能通过Bax和caspase-3介导。Aim To study the effect induced by three cyclic hexadepsipeptides isolated from fungi and to explore its mechanism.Method Human PC-3 cells were cultured in vitro and then treated with cyclic hexadepsipeptides at the concentration of 10 μmol·L-1.The effect of three cyclic hexadepsipeptides on human PC-3 cells was measured by MTT assay.The apoptosis of the cells was evaluated by TUNEL assay.The protein expression of apoptosis in PC-3 cells was analyzed by Western blot assay.Results After three cyclic hexadepsipeptides（10 μmol·L-1） treatment,Pullularin C showed significant activities against human PC-3 cells;Cell apoptosis of Pullularin C treated group was significantly higher than that in control group,and which also significantly up-regulated the Bax and caspase-3 protein expression compared with control group in PC-3 cells.Conclusions Pullularin C has a significant inhibitory effect on the protiferation of human PC-3 cells.Besides,Pullularin C can also induce cells apoptosis through the mediation of Bax and caspase-3.

关 键 词：环六缩酚肽 前列腺癌细胞 抑制活性 细胞凋亡Bax CASPASE-3 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]