SiRNA沉默JAB1表达抑制肝癌细胞增殖  

SiRNA silencing JAB1 expression inhibit hepatoma cells proliferation

作　　者：程晓刚[1] 叶琬 张绍兰[1] 陈玮[1] 金虹[1] 徐艳[1] 李晋川[1] 

机构地区：[1]成都医学院病原学教研室,四川成都610083 [2]成都蓉生药业有限公司,四川成都610041

出　　处：《基础医学与临床》2012年第11期1288-1292,共5页Basic and Clinical Medicine

基　　金：四川省教育厅应用基础研究项目(10ZC072)

摘　　要：目的观察沉默JAB1基因表达对人肝癌细胞HepG-2增殖的影响。方法利用RNA干扰技术构建pAVU6-JAB1 siRNA干扰质粒,将其转染HepG-2细胞,采用real-time PCR、Western blot等方法对JAB1表达进行检测,WST-8、流式细胞分析对细胞增殖的影响。结果成功构建pAVU6-JAB1 siRNA干扰质粒,转染HepG-2细胞后JAB1蛋白表达量较对照组明显降低,P27kip1蛋白表达量明显升高(P<0.01)。转染96 h后能显著抑制细胞增殖活性,由空载体转染组的99.6%±1.4%降至70.8%±1.6%。结论构建靶向JAB1基因的干扰质粒能下调JAB1基因的表达,上调P27kip1蛋白水平,并抑制HepG-2细胞在体外的增殖活性。Objective Observe the effect of silencing JAB1 gene expression on human hepatoma cells proliferation.Methods pAVU6-JAB1 siRNA interference vectors were constructed and transfected into hepatoma cell line HepG-2.The JAB1 expression was measured using real-time PCR and weatern-blot methods.The cell proliferation was measured using WST-8 methods.Results 96 hours after transfection of the pAVU6-JAB1 siRNA vectors,the proliferation of hepatoma cell line was inhibited from 99.6%±1.4% to 70.8%±1.6%（experimental group）.JAB1 expression was also inhibited,whereas the P27kip1 expression level was significantly elevated.Conclusions Inhibition of the expression of the JAB1 protein in hepatoma cells may inhibit hepatoma cell proliferation to some extent.

关 键 词：JAB1 RNA干扰 肝癌细胞 增殖 

分 类 号：R735.7[医药卫生—肿瘤]