脉冲强磁场对新生大鼠神经干细胞β－连接素表达的影响  

The effects of a high-intensity pulsed electromagnetic field on the expression of β-catenin in the neural stemcells of neonatal rats

作　　者：邹丽丽[1] 许涛[1] 龙兴蓝[1] 石磊[2] 彭涛[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院康复科,武汉430030 [2]华中科技大学同济医学院附属同济医院骨科,武汉430030 [3]华中科技大学脉冲强磁场中心

出　　处：《中华物理医学与康复杂志》2012年第10期729-732,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金（面上项目）资助（81271732）

摘　　要：目的通过检测神经干细胞B一连接素基因和蛋白的表达，初步探讨脉冲强磁场（0．1Hz，4T，8次）促神经于细胞增殖的作用机制。结果取新生SD大鼠双侧脑室下区神经干细胞，无血清培养2周，将细胞分成实验组和对照组。实验组的细胞给予0．1Hz4T的脉冲强磁场干预8次；对照组的细胞置相同条件下不行干预。此后第1、3、5和7天，分别用RT—PCR和Westernblot检测两组神经干细胞B一连接素的表达。结果干预后第1、3、5和7天，RT-PCR法显示实验组神经干细胞β一连接素基因表达较对照组明显增高（P〈0．05）；Westernblot也显示实验组神经干细胞β一连接素蛋白表达较对照组明显增高，增高水平在干预后第7天最显著。结论新生大鼠神经干细胞经0．1Hz，4T脉冲强磁场干预8次后，β一连接素在基因及蛋白水平均增高，提示脉冲强磁场（0．1Hz，4T，8次）促神经千细胞增殖的机制可能是通过激活Wnt／13一连接素通路实现。Objective To study the mechanism by which a high-intensity pulsed electromagnetic field （HIPEMF） （0.1 Hz, 4 T, 8 pulses） facilitates the proliferation of neural stem cells by detecting the expression of β- catenin genes and protein. Methods Neural stem ceils （NSCs） were isolated from the sub-ventricular zone （SVZ） of neonatal rats and cultured in supplemented, serum-free medium for two weeks. The NSCs were then divided into an experimental group exposed to a HIPEMF for 8 pulses and a control group given sham stimulation. The gene and pro- tein expression of β-catenin in the NSCs were assayed by RT-PCR and Western blotting on the 1 st, 3rd, 5th and 7th day after the stimulation. Results The NSCs＇ cIoned spheres were round and translucent, and showed red fluores- cence after staining with anti-nestin （cy3）. The RT-PCR results showed β-catenin genes were highly expressed in the exposed group （significantly more than in the controls）. The Western blotting showed that expression of β-catenin protein was also higher in the experimental group, especially at the 7th day after stimulation, a difference which was also statistically significant. Conclusion HIPEMF at 0.1 Hz, 4 T, in 8 pulse trains can promote NSC prolifera- tion, perhaps through the Wnt/β-catenin signaling pathways.

关 键 词：脉冲强磁场 神经干细胞 增殖 机制 Wnt/β一连接素 

分 类 号：R[医药卫生]