不同强度激光照射对溃疡性结肠炎大鼠炎症反应的影响  

The effect of laser irradiation on oxazolone-induced ulcerative colitis

作　　者：李猛[1] 杜金刚[2] 黄力平[1] 刘畅格[1] 韩玉山[2] 韩秀娟[2] 

机构地区：[1]天津体育学院健康与运动科学系,天津300381 [2]天津市人民医院康复医学科

出　　处：《中华物理医学与康复杂志》2012年第10期737-741,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的采用嗯唑酮制作溃疡性结肠炎（UC）大鼠模型，观察不同强度激光照射对UC大鼠病理性炎症反应的影响，并探讨其可能作用机制。方法共选取成年健康雄性SD大鼠30只，采用随机数字表法将其分为正常组（6只）、UC对照组（8只）、200mW激光组（8只）及400mW激光组（8只）。除正常组以外，其它大鼠均采用嗯唑酮将其制成UC大鼠模型。两激光组大鼠于制模后分别给予相应功率激光照射，每天照射1次，每次照射10mm，连续照射10d。每次激光照射结束后观察各组大鼠UC疾病活动指数（DAI），然后处死大鼠提取结肠组织制成标本，通过肉眼观察结肠病变并进行大体形态学损伤评分，计算结肠湿质量指数；另外取结肠标本行病理切片检查，观察激光照射后黏膜各层炎症、水肿及细胞浸润情况。结果与UC对照组比较，400mw激光组大鼠体质量显著提升（P〈0．05），接近正常组体重（P〉0．05），DAI评分显著下降（P〈0．05），黏膜上皮、黏膜固有层及黏膜下层厚度基本恢复正常（P〉0．05），组织学损伤评分显著好转（P〈0．05），结肠湿质量指数明显降低（P〈0．01）；病理检查发现黏膜溃烂、黏膜下水肿、炎性细胞浸润等现象均显著缓解，仅有少量炎性细胞浸润；而200mW激光组经激光照射后，其上述各项指标均较UC对照组明显好转（P〈0．05），但仍不及400mW激光组恢复水平。结论一定功率强度的激光照射对UC大鼠具有治疗作用，可显著减轻大鼠结肠组织病理性炎症反应，以患部黏膜下层水肿的改善情况尤为明显。Objective To observe any therapeutic effect of laser irradiation on pathological inflammatory re actions in ulcerative colitis （UC） induced by oxazolone, and to investigate possible mechanisms. Methods Six rats were selected as a normal control group. Another 24 rats with UC induced by oxazolone were randomly assigned to a UC model group （ n = 8 ） , a 400 mW laser group （ 161. 3 mW/cm2 , n = 8 ） , and a 200 mW laser group （80.6 mW/cm2 , n = 8）. All the rats were fixed in custom-buih devices. Those in the therapy groups were treated daily, 10 rain per time, for 10 days. After the end of the last irradiation session, disease activity indexes （DAIs） were observed. Rats from every group were sacrificed 24 h after the last irradiation in order to observe any pathologi cal changes in colon tissue, the weight of fresh ulcerated tissues, and gross changes in morphology. Colon segments were stained with hematoxylin-eosin and histological lesion scoring was performed under a light microscope. Any changes in inflammatory edema in the colonic mucous membrane were observed before and after laser irradiation. Results The UC mode[ was successfully established. Average body weight in the 400 mW laser group increased sig- nificantly more than in the UC model group, approaching that of the normal control group. DAI decreased significant- ly. The thickness of the epithelial mucous membrane, lamina propria and submucosa was basically restored. Histolog- ical lesion scores also improved significantly, and the weight of fresh ulcerative tissue was significantly lower. Mucous membrane ulcers, submucosa edema and inflammatory cell infiltration all were alleviated significantly, merely presen- ting a few inflamed cells and small amounts of per[phlebitis. In the 200 mW laser group all these outcome measures improved significantly compared with the UC model group, but were not as good as in the 400 mW laser group, and the rats needed longer to recover. Conclusion Laser irradiation at 400 mW has advantages over 200 mW, a

关 键 词：激光 噁唑酮 溃疡性结肠炎 

分 类 号：R574.62[医药卫生—消化系统]