消化道不同恶性肿瘤患者血清锌α-2-糖蛋白水平检测及临床意义  

Detecting the expression of serum zincα -2-glycoprotein in patients with various digestive system carcinoma and its clinical significance

作　　者：孟倩[1] 赵旭宏[1] 赵瑾[1] 雷婷[1] 张曼[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院临床检验中心,北京100038

出　　处：《首都医科大学学报》2012年第5期665-668,共4页Journal of Capital Medical University

基　　金：北京市自然科学基金(7122086)~~

摘　　要：目的对脂肪降解中起重要作用的脂质降解因子锌α-2-糖蛋白(zinc-α-glycoprotein,ZAG)在消化道不同恶性肿瘤患者血清中的表达进行了研究,旨在探讨这些肿瘤患者的脂质代谢状态与ZAG的关系。方法实验采集了不同消化道恶性肿瘤患者、相应良性病变及正常健康人血清样本,每种疾病及正常血清样本各6例,检测了所有样本的脂代谢相关指标。采用蛋白免疫印迹的方法对血清中锌α-2-糖蛋白(zinc-α-glycoprotein,ZAG)的含量进行了半定量鉴定。结果在各疾病组中,总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)在血清中的含量在健康对照组、良性组、恶性组间差异无统计学意义。在胃病组、结肠疾病组、直肠疾病组中,锌α-2-糖蛋白(ZAG)在恶性、良性和正常组中的表达不具有统计学意义(P=0.589,P=0.618,P=0.864)。而ZAG在肝癌患者血清中的表达与正常健康人、良性组相比,差异具有统计学意义(P=0.013,P=0.008),但正常健康人与良性组患者比较,差异无统计学意义(P=0.804)。结论锌α-2-糖蛋白(ZAG)在消化道不同肿瘤患者血清中的表达差异有统计学意义,肝癌组相对于胃癌组、结直肠癌组的表达具有临床诊断意义。与总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)相比,它在评价肝癌患者脂代谢状态方面更加灵敏。Objective To explore the relationship between zinc-α-2-glycopmtein （ZAG） and lipid metabolic state of tumor patients, the serum levels of lipolytic factor ZAG in patients with various digestive system carcinoma were detected. Methods We collected serum samples from patients with various digestive system carcinoma, benign lesions and normal subjects. For each disease and normal subject we collected 6 cases. And then we detected lipid metabolism related indexes of all samples. The serum levels of ZAG were semi-quantitative identified by using western blotting. Results In each group, the serum levels of TC, TG, HDL-C, LDL-C were no significant difference among the normal, benign and malignant groups. In gastric, colonic, rectal groups, the expression of ZAG in serum among the normal, benign and malignant groups had no obvious difference （P =0.589, P =0.618, P =0. 864）. However,there was a significant difference between hepatocellular carcinoma group and normal group or benign group （P =0. 013, P =0.008）, but between the normal group and benign group, there was no statistical significance （P =0. 804）. Conclusion The expression of ZAG in serum were different among various digestive system carcinoma,especially, hepatocellular carcinoma group was more significant than gastric, colonic and rectal malignant groups. Compare with TC, TG, HDL-C and LDL-C, ZAG may be a more sensitive indicator for evaluating lipid metabolism of patients with hepatocellular carcinoma.

关 键 词：锌α-2-糖蛋白 胃癌 肝细胞癌 结肠癌 直肠癌 脂代谢 

分 类 号：R730.4[医药卫生—肿瘤]