鸡源性肠炎沙门氏菌的鉴定  

Identification of Salmonella enteritidis strain from chicken

作　　者：王涛[1,2] 王亮[1] 杜冬冬[1] 庄燕飞[1] 常维山[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]秦皇岛出入境检验检疫局,秦皇岛066004

出　　处：《中国人兽共患病学报》2012年第10期982-986,共5页Chinese Journal of Zoonoses

基　　金：国家标准委员会全国动物防疫标准化技术委员会项目鸡肠炎沙门氏菌PCR检验技术规范资助项目(20110221-T-326)~~

摘　　要：目的运用多种方法鉴定临床分离的1株沙门氏菌。方法从细菌培养特性、生化特性方面来鉴定临床分离菌属于那类细菌;测定临床分离菌的血清型根据血清型试验结果进一步确定临床分离菌的类型;测定临床分离菌的16srRNA从分子生物学角度鉴定细菌;应用多位点序列分型(MLST)对该株细菌进行分型。结果从临床分离的沙门氏菌与肠炎沙门氏菌的微生物学和生物化学特征相符合,血清学试验结果确定其血清型为O3,19(+);O9(+);Hg,p(+);Hg(+);Hm(+),16sRNA基因测序结果显示该株细菌与鸡肠炎沙门氏菌P125109同源性达100%,多位点序列分型结果显示该株细菌基因型为ST11。结论该株细菌为肠炎沙门氏菌血清型为O3,19(+);O9(+);Hg,p(+);Hg(+);Hm(+),基因型为ST11。The purpose of this test is that to determine the type of a clinical isolated strain of Salmonella.The strain was classified by cultural characteristics analysis,biochemical methods,serological identification and molecular biological method,and was typed by multilocus sequence typing（MLST） method.The results showed that the clinical isolated strain was consistent with microbiological characteristics of Salmonella enteritidis.Results of serological identification proved that the serotype of the clinical isolated strain were O3,19（＋）;O9（＋）;Hg,p（＋）;Hg（＋）;Hm（＋）.Sequencing result of 16s RNA gene showed that the nucleotide sequence homology of the clinical isolated strain was 100% comparing with that of Salmonella enteritidis P125109.Result of MLST revealed that the genotype of the clinical isolated strain was ST11.The clinical isolated strain was confirmed as Salmonella enteritidis,whose serotype was O3,19（＋）;O9（＋）;Hg,p（＋）;Hg（＋）;Hm（＋）,and the genotype was ST11.

关 键 词：肠炎沙门氏菌 分类 多位点学列分型 基因型 

分 类 号：R378.2[医药卫生—病原生物学]