熊果酸对二乙基亚硝胺诱发小鼠肝癌前病变的防护作用  

Effect of ursolic acid on DEN-induced hepatic precancerous lesions in mice

作　　者：毛文超[1] 宋艺君[1] 张健[1] 焦艺博[1] 冯丽莉[1] 白荟芳[1] 王秀萍[1] 蔡大勇[2] 王玥琦[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]中国医学科学院药用植物研究所

出　　处：《中西医结合肝病杂志》2012年第5期287-289,292,I0005,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：北京中医药大学创新团队项目(No.2011-CXD-04);重大新药创新科技重大专项(No.2009ZX09502-017);教育部重点研究资助项目(No.108019)

摘　　要：目的:观察熊果酸和熊果酸联合枸杞对小鼠肝癌前病变的防护作用。方法:将40只小鼠分为4组:正常组、模型组、熊果酸组和联合用药组,每组10只。除正常组外其余3组小鼠均每周腹腔注射1次二乙基亚硝胺复制小鼠肝癌前病变模型,同时正常和模型组小鼠每天灌胃生理盐水,熊果酸组小鼠每天灌胃熊果酸,联合用药组小鼠每天灌胃熊果酸和枸杞子,均连续10周。腹主动脉取血检测血清肝功能指标,固定光镜观察肝组织学变化。结果:与对照组比较,模型组小鼠肝脏同时存在变质与修复性病变,增生肝细胞具有异型性,血清ALT、AST、ALP明显升高(P<0.01),GST升高(P<0.05)。熊果酸组小鼠肝损伤较轻,增生肝细胞轻度异型性,ALT、AST、GST较模型组显著降低(P<0.01),ALP降低(P<0.05);联合用药组小鼠ALT、AST较模型组显著降低(P<0.01),GST降低(P<0.05),ALP有降低的趋势(P>0.05)。结论:熊果酸对诱导性小鼠肝癌前病变具有防治作用;熊果酸与枸杞联合应用疗效更好。Objective：To observe the effect of ursolic acid on hepatic precancerous lesion in mice.Methods：Except for rats in control,model rats were intraperitoneally injected with diethylnitrosamine（DEN） for inducing hepatic precancerous lesions.Ursolic acid（225.0mg/kg） or it（112.5 mg/kg） with medlar Abstraction（1 200.0mg/kg） was administrated intragastricall for treating the lesions in 10 weeks.Hepatic function was detected as ALT,AST,ALP,GST.Pathological changes were observed under microscopy and their degrees were evaluated by morphormetry.Results：Compared with those of control rats,the hepatic precancerous lesions of model rats were indicated as the hepatic dysfunction as elevated ALT,AST,ALP activities in sera,the hepatic cirrhosis as decreased hepatic indexes,collagen deposition and pseudolobule formation,and the atypia of hepatocytes as the increased nuclear volloms.The lesions in model rats were confirmed with their morphormetries.The pathological changes were relived with ursolic acid,more with the ursolic acid jointed with medlar Abstraction.Conclusion：Ursolic acid has the potency to relieve hepatic precancerous lesions.This effect was enhanced with medlar.

关 键 词：熊果酸/药理作用 二乙基亚硝胺 肝癌前病变 枸杞子/药理作用 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]