1,2-萘醌-4-磺酸钠分光光度法测定甘氨酸  

Spectrophotometric determination of glycine using 1,2-naphthoquinone-4-sulfonic acid sodium

作　　者：占海红[1] 乔月纯[2] 李全民[3] 

机构地区：[1]平顶山教育学院生化系,河南平顶山467000 [2]河南机电高等专科学校电气工程系,河南新乡453002 [3]河南师范大学化学与环境科学学院,河南新乡453007

出　　处：《河南城建学院学报》2012年第5期27-29,35,共4页Journal of Henan University of Urban Construction

摘　　要：建立了用1,2-萘醌-4-磺酸钠测定甘氨酸的新方法。研究表明:1,2-萘醌-4-磺酸钠与氨基酸在pH为10.0的缓冲溶液中反应,生成组成比为1∶1的浅红色产物,最大吸收波长λmax=474 nm,表观摩尔吸光系数ε=2.8×103L.mol-1cm-1。甘氨酸浓度在0.4～80 mg/L范围内呈良好的线性关系,线性回归方程为A=0.017 64+0.013 34c(mg/L),线性相关系数r=0.999 3,检测限为0.12mg/L,R.S.D为0.64﹪,平均回收率为99.7%以上。本法用于测定药物样品中甘氨酸的含量,结果满意。A new method for the determination of glycine by using 1,2-naphthoquinone-4-sulfonic sodium（NQS） is established.The method is based on the pink product formed from glycine with NQS in a buffer solution of pH 10.0,and the stoichiometric ratio of the product is 1∶ 1.Beer＇s law is obeyed in a range of 0.4~80 mg·L-1 of glycine at the maximum absorption of 474nm（ε=2.8×103 L·mol-1cm-1）.The linear regression equation of the calibration graph is A=0.01764＋0.25291C,with a linear regression correlation coefficient of 0.9993.The detection limit is 0.2 μg·mL-1,R.S.D.is 0.64% and average recovery is over 99.7%.This proposed method has been successfully applied to the determination of glycine with satisfactory results.

关 键 词：分光光度法 氨基酸 1 2-萘醌-4-磺酸钠 化学衍生 

分 类 号：O657[理学—分析化学]