载脂蛋白E拟肽对EAE小鼠的髓鞘和轴突损伤的影响  

Effect of apolipoprotein E-mimetic peptides on demyelination and axonal injury in mice with experimental autoimmune encephalomyelitis

作　　者：韦俊杰[1] 郑明华[1] 梁培霄[1] 陶敏[1] 唐玉兰[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021

出　　处：《中风与神经疾病杂志》2012年第10期872-875,共4页Journal of Apoplexy and Nervous Diseases

基　　金：广西自然科学基金资助项目(No.2012GXNSFAA053082);广西自然科学基金资助项目(桂科回0991009)

摘　　要：目的探讨载脂蛋白E(ApoE)拟肽对实验性自身免疫性脑脊髓炎(EAE)小鼠中枢神经系统髓鞘脱失和轴突损伤的影响。方法以髓鞘少突胶质细胞糖蛋白多肽(MOG35-55)为抗原建立EAE模型。将40只雌性C57BL/6J小鼠随机分为4组,即正常组、EAE组、正常治疗组和EAE治疗组,两个治疗组皮下注射ApoE拟肽。免疫组化法检测髓鞘碱性蛋白(MBP)和神经丝轻链(NF-L)的表达。结果 EAE治疗组中脑和脊髓的MBP和NF-L的表达均高于EAE组(P<0.05)。结论 ApoE拟肽可能对EAE的髓鞘和轴突的损伤有保护作用。Objective To explore the effect of Apolipoprotein E-mimetic peptides on demyelination and axonal injury in central nervous system of mice with experimental autoimmune encephalomyelitis.Methods 40 female C57BL/6J mice were randomly divided into 4 groups.EAE model was induced in mice by immunized with myelin oligodendrocyte glycoprotein peptides（MOG35-55）.ApoE-mimetic peptides was injected subcutaneously in two treatment groups.The adjuvant group and EAE group was injected with saline.The expression of MBP and NF-L was detected by immunohistochemistry.Results The expression of MBP and NF-L in the EAE group was more than the EAE treatment group in every part except brain（P〈0.05）.Conclusion The ApoE-mimetic peptides may have protective effect of demyelination and axonal injury.

关 键 词：载脂蛋白E 多发性硬化 实验性自身免疫性脑脊髓炎 髓鞘碱性蛋白 神经丝轻链 

分 类 号：R742[医药卫生—神经病学与精神病学]