卒中后抑郁大鼠小脑P38、BDNF改变的实验研究  

Study on cerebellar functional changes in rats with post-stroke depression

作　　者：刘欣跃[1] 王东玉[2] 隋汝波[3] 

机构地区：[1]辽宁医学院,辽宁锦州121000 [2]辽宁医学院第五临床学院(锦州市中心医院)神经内科,辽宁锦州121000 [3]辽宁医学院附属第一医院神经内科,辽宁锦州121000

出　　处：《中风与神经疾病杂志》2012年第10期886-889,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金项目(81041058)

摘　　要：目的观察卒中后抑郁(post-stroke depression,PSD)模型大鼠小脑功能的改变。方法选用健康雄性SD大鼠,随机分为4组,对照组、卒中组、单纯抑郁组、卒中后抑郁组(PSD组);并分7d、14d、21d 3个时间点进行行为学观察和测试、免疫组化法测定小脑组织功能活性依赖蛋白突触素(P38)、脑源性神经营养因子(BDNF)的表达。结果与卒中组相比,单纯抑郁组大鼠体重和糖水消耗量下降,水平运动及垂直运动水平降低(P<0.01);PSD组大鼠体重和糖水消耗量下降更为明显,水平运动及垂直运动水平降低也最为明显(P<0.01)。与卒中组比较,单纯抑郁组P38、BDNF的表达普遍减弱,与单纯抑郁组相比较,PSD组P38、BDNF的表达减弱的更为明显;组内不同时间点(7d、14d、21d)进行比较:卒中组、单纯抑郁组、PSD组随着时间点的推移,小脑组织P38、BDNF表达也呈现出减弱的趋势。结论大鼠小脑功能的改变可能与PSD的发生有关,进一步研究可能有助于阐明卒中后抑郁症的发病机制。Objective To observe the changes of cerebellar function in the post-stroke depression（PSD） model of rats.Methods The healthy SD rats were randomly divided into four groups,sham group,stroke group,depression group,and PSD group.Behavioral observation and testing were performed and the expression of P38 and BDNF in cerebellum by immunohistochemical method of on 7days,14days and 21days were detected.Results Compared with stroke group,depression rats,weight and sugar water consumption were decreased,horizontal motion and vertical motion level were lower（P〈0.01）;PSD rats,weight and sugar water consumption drop were more apparent,horizontal motion and vertical motion level also reduced the most obviously（P〈0.01）.Group comparison：Compared with the stroke group,in depression group and PSD group,the expression of P38 and BDNF in cerebellum were decreased.The difference between them was very significant.Intergroup comparison（7d,14d,21d）：Compared with the stroke group,in depression group and PSD group on 7d,14d and 21d,P38 and BDNF were significantly differencedifferent.HE staining showed neuronal cell damage.Conclusion Cerebellar dysfunction has the potential to be involved in PSD.

关 键 词：卒中后抑郁 小脑 功能活性依赖蛋白突触素P38 脑源性神经营养因子 

分 类 号：R749[医药卫生—神经病学与精神病学]