泌尿生殖道沙眼衣原体慢性持续感染患者人类白细胞抗原DQB1等位基因多态性研究  

Polymorphism of HLA-DQB1 alleles in Chinese Han patients with persistent Chlamydia trachomatis genital infection

作　　者：刘志超[1,2] 刘原君[1] 江勇[3] 陈立新[1] 刘全忠[1] 

机构地区：[1]天津医科大学总医院皮肤性病科,天津300052 [2]济宁医学院临床学院,山东济宁272067 [3]天津医科大学附属第二医院,天津300052

出　　处：《中国病原生物学杂志》2012年第10期747-749,共3页Journal of Pathogen Biology

基　　金：山东省自然科学基金项目(No.ZR2011HL009)

摘　　要：目的探讨人类白细胞抗原(HLA)-DQB1等位基因多态性与泌尿生殖道沙眼衣原体慢性持续感染的遗传易感相关性。方法应用聚合酶链反应-序列特异性引物(PCR-SSP),分别对泌尿生殖道沙眼衣原体慢性持续感染患者、一般感染患者和正常人各80例,进行HLA-DQB1等位基因分型,分析HLA-DQB1等位基因与泌尿生殖道沙眼衣原体慢性持续感染的相关性。结果泌尿生殖道沙眼衣原体慢性持续感染患者中HLA-DQB1*0602等位基因频率为45%,一般感染组为15%,健康对照组为22.5%,差异有统计学意义(χ2=14.08,P<0.01)。结论 HLA-DQB1*0602可能是沙眼衣原体泌尿生殖道慢性持续感染的易感基因或与致病基因相连锁;PCR-SSP可用于快速检测HLA-DQB1等位基因型。Objective To analyze the association between HLA-DQB1 gene polymorphism and persistent genital Chlamydia trachomatis infection.Methods HLA-DQB1 alleles from 80 patients with a persistent genital Chlamydial infection,80 patients with a typical genital Chlamydial infection,and 80 normal human controls were genotyped using polymerase chain reaction-sequence-specific primers（PCR-SSP）.Result The frequency of the HLA-DQB1＊0602 allele was 45% in patients with a persistent genital Chlamydial infection,15% in patients with a typical genital Chlamydial infection,and 22.5% in normal human controls.The frequency differed significantly（χ2=14.08,P0.01）.Conclusion The HLA-DQB1＊0602 allele may be a susceptibility gene or may be closely linked to genes for susceptibility to persistent genital Chlamydial infection.PCR-SSP is a rapid and economical method for genotyping HLA-DQB1 alleles and can be used routinely.

关 键 词：沙眼衣原体 慢性持续 HLA-B1抗原 HLA-DQ抗原 等位基因 

分 类 号：R374.1[医药卫生—病原生物学]