HLA-E在肝癌细胞系中的表达  

Expression of human leukocyte antigen E in hepatocellular carcinoma cell lines

作　　者：张彤[1] 曾宪成 李华[1] 陈文捷[1] 方天翎[3] 聂常富 陈规划[1] 

机构地区：[1]中山大学附属第三医院肝移植中心,广东广州510630 [2]增城市人民医院普通外科,广东广州511300 [3]广州医学院第一附属医院肝胆外科,广东广州510120 [4]郑州大学附属肿瘤医院肝胆外科,河南郑州450003

出　　处：《中国病理生理杂志》2012年第10期1884-1886,共3页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展计划(973分课题)(No.2009CB522404);国家自然科学基金面上项目(No.30571769;No.30972914);十一五科技攻关(No.2008ZX10002-025);广东省科技计划(No.2009A030200006);教育部新教师基金资助项目(No.20100171120087;No.20100171120084);广东省自然科学基金博士启动项目(No.10451130001004472)

摘　　要：目的:研究人类白细胞抗原E(HLA-E)在肝癌细胞系中的表达情况。方法:通过real-time PCR和Western blotting技术研究5种肝癌细胞系和胎肝细胞系中HLA-E mRNA和蛋白的表达情况,进行相对定量分析。结果:Real-time PCR检测显示,HepG2细胞、Bel7402细胞、PLC细胞、MHCC97细胞和Hep3B2.1-7细胞这5种肝癌细胞系与L02胎肝细胞系的HLA-E mRNA水平比较,除Hep3B2.1-7细胞表达几乎接近缺失(P<0.01)外,其余无显著差异(P>0.05)。HepG2细胞、Bel7402细胞、PLC细胞、MHCC97细胞和Hep3B2.1-7细胞与L02细胞的HLA-E蛋白水平比较,差异显著(P<0.01),其中Hep3B2.1-7细胞表达缺失。结论:肝癌细胞系的HLA-E mRNA和蛋白表达不同步,可能存在转录后调控。AIM： To investigate the human leukcyte antigen E （HLA- E） expression in hepatocellular carci- noma cell lines. METHODS： The techniques of real - time PCR and Western blotting were used to study the HLA - E ex- pression in the 5 cell lines of hepatocellular carcinoma and a fetal liver cell line at mRNA and protein levels. RESULTS ： The results of real - time PCR showed that no statistical difference of HLA - E mRNA level between fetal liver cell L02 and other 4 cell lines of hepatocellular carcinoma （ HepG2, Be17402, PLC and MHCC97 ） was observed, and almost absence of HLA- E mRNA expression in Hep3B2.1 -7 cells was detected. However, the results of Western blotting showed that there was a significant statistical difference of HLA - E protein levels between L02 cells and the 5 cell lines of hepatocellular carcinoma （HepG2, Be17402, PLC, MHCC97 and Hep3B2.1 -7）, and no HLA - E protein in Hep3B2.1 -7 cells was detectable. CONCLUSION： Asynchronization of HLA - E expression between mRNA and protein levels was found in hepatocellular carcinoma cell lines.

关 键 词：人类白细胞抗原E 肝细胞癌 胎肝细胞 基因表达 

分 类 号：R730.5[医药卫生—肿瘤]