万寿菊提取物的安全性毒理学试验研究  

Toxicological assessment of marigold extract

作　　者：赵珺彦[1] 翟鹏贵[2] 周大兴[2] 郑勇英[3] 

机构地区：[1]浙江省杭州市萧山区第一人民医院,杭州311200 [2]浙江中医药大学药学院,杭州310053 [3]上海市疾病预防控制中心,上海200336

出　　处：《中国卫生检验杂志》2012年第10期2373-2375,2378,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:为万寿菊提取物安全性评价提供毒理学依据。方法:按卫生部《保健食品检验与评价技术规范》(2003年版)进行小鼠急性毒性试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验、Ames试验和大鼠30 d喂养试验。结果:万寿菊提取物对雌雄小鼠急性经口的最大耐受剂量(MTD)均大于20.0 g/kg BW,属无毒级;三项致突变试验均未见致突变作用;大鼠30 d喂养试验结果,3个剂量组大鼠各项指标与对照组比较均无显著性差异。结论:万寿菊提取物属无毒级;未见潜在致突变作用;大鼠30 d喂养试验,未见明显毒性反应。Objective： To provide basis for the toxicological assessment of marigold extract. Methods ： Acute tox- icity test, mice bone marrow cell micronucleus test, mice sperm abnormality test, Ames test and thirty - day - feed- ing study were conducted according to methods in ＂Standard for Testing and Assessment of Health Food＂. Results： The acute oral MTDs of marigold extract on mice were all greater than 20.0 g/kg BW, belonging to non - toxic grade. Marigold extract in the bone marrow cell micronucleus test, mice sperm abnormality test and Ames test were all not found mutation effect. Thirty -day -feeding test on rats showed that there was no significant difference be- tween the control group and the other three dosage groups respectively. Conclusion： Marigold extract was non - tox- ic without potential mutagenicity. No obvious toxic reaction was found in thirty -day - feeding test of rats.

关 键 词：万寿菊提取物 急性毒性试验 微核试验 精子畸形试验 AMES试验 30d喂养试验 

分 类 号：R965.2[医药卫生—药理学]