一起由维尔肖沙门菌引起的食物中毒事件的病原溯源  

Investigation on a food poisoning event due to Salmonella virchow

作　　者：张雯霞[1] 陈敏[1] 张曦[1] 陈洪友[1] 席曼芳[1] 王刚毅[1] 

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《中国卫生检验杂志》2012年第10期2393-2395,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:运用基因分型技术对一起由维尔肖沙门菌引起的食物中毒进行溯源分析,探讨分子分型技术在沙门菌食物中毒中病原菌溯源和应用可能性。方法:采用脉冲场凝胶电泳(PFGE)法对一起食物中毒事件中分离的11株维尔肖沙门菌进行基因分型和溯源性分析。结果:从病人肛拭中分离出的8株维尔肖沙门菌和引起该食物中毒的食物中分离的3株菌株都属于同一克隆。结论:此次食物中毒由同一株维尔肖沙门菌引起,PFGE分型技术可以直观的判断分离菌的亲缘关系,为流行病学调查和溯源提供可靠而实际的依据。Objective： To investigate the pathogens of a food poisoning event by the method of genotyping, to diss- cuss the application possibilities of genotyping in traceability of the pathogens of Salmonella from poisoning food. Methods： Pulsed - field gel electrophoresis （PFGE） was used to analyze 11 isolates of Salmonella Virchow separa- ted from the food poisoning event. Results： Eight strains isolated from patients and three strains isolated from foods were all the same. Conclusion ： The food - poisoning is caused by the same genotype of S. Virchow strains. PFGE methods is helpful to find out the heredity background of isolated bacteria and to provide reliable and practical source of epidemiological investigation.

关 键 词：食物中毒 维尔肖沙门菌 脉冲场凝胶电泳 

分 类 号：R378.22[医药卫生—病原生物学]