结膜瓣覆盖治疗角膜碱烧伤中MMP-9和TIMP-1的表达  

Expression of MMP-9 and TIMP-1 in alkali-burned cornea of conjunctival flap covering

作　　者：宋东宇[1,2] 高明宏[1] 徐旭[1] 于静[1] 禹海[1] 陈颖新[1] 张晓光[1] 

机构地区：[1]沈阳军区总医院眼科,中国辽宁省沈阳市110016 [2]朝阳市中心医院眼科,中国辽宁省朝阳市122000

出　　处：《国际眼科杂志》2012年第11期2062-2065,共4页International Eye Science

基　　金：中国辽宁省自然科学基金资助项目(No.20102252)~~

摘　　要：目的:研究结膜瓣覆盖术治疗兔角膜碱烧伤中基质金属蛋白酶-9(MMP-9)及组织型基质金属蛋白酶抑制剂-1(TIMP-1)的表达机制。方法:将50只兔随机分成实验组及对照组,每组分别25只,建立兔角膜烧伤模型。实验组在角膜烧伤后当天行结膜瓣覆盖术。采用免疫组化法测定两组角膜碱烧伤后不同时间点MMP-9和TIMP-1的表达。结果:MMP-9在角膜碱烧伤后的3d开始升高,14d达到最高,之后逐渐下降;而TIMP-1在伤后即有表达,7d有所下降,于14d达到最低,21d达到峰值。实验组MMP-9的表达均明显低于对照组,且角膜碱烧伤后的3,14,21d和28d差异有统计学意义(P<0.05);而TIMP-1则明显高于对照组,且角膜碱烧伤后3,14d和21d差异有统计学意义(P<0.05)。结论:角膜碱烧伤的病理损伤及修复过程中,MMP-9及TIMP-1的表达密切相关。结膜瓣覆盖治疗重度角膜碱烧伤的疗效确切。AIM：To study the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor metalloproteinase-1（TIMP-1） after conjunctival flap covering in alkali-burned cornea.METHODS：Rabbit cornea alkali-burns model was made,then 50 rabbits were randomly divided into experimental group（n=25） and control group（n= 25）.At the same time the surgery of conjunctival flap covering was given to rabbits of the experimental group.The condition developing of alkali-burned cornea was observed by slit-lamp biomicroscopy,photography in two groups,and the expression of MMP-9 and TIMP-1 was identified by immunohistochemisty in different period.RESULTS：：Expression of MMP-9 increased on the 3rd day,shown a peak on the 14th day,and then decreased gradually.TIMP-1 expressed in the early phrase,and later,the level decreased on 7th day.TIMP-1 level reached the lowest level on 14th day,then increased and shown a peak on the 21st day.MMP-9 level of the experimental group was significantly lower than that of the control group（the 3rd,14th,21st,and 28th day）,while TIMP-1 level（the 3rd,14th and 21st day） was higher（P0.05）.CONCLUSION：During the wound healing process,alkali-burned cornea has close relation with the expression of MMP-9 and TIMP-1.The treatment of conjunctival flap covering for the severe alkali-burned cornea was found to have good effect.

关 键 词：结膜瓣 角膜碱烧伤 基质金属蛋白酶-9 组织型基质金属蛋白酶抑制剂-1 

分 类 号：R779.1[医药卫生—眼科]