腺病毒介导D2S基因联合溴隐亭对人垂体无功能腺瘤细胞生长的抑制作用  

Inhibitory effect of adenovirus-mediated D2S gene plus bromocriptine therapy on primary cultures of human non-functioning pituitary adenoma cells

作　　者：熊振坤[1] 李伟强[1] 刘洁[1] 吴哲褒[1] 李强[1] 陈云祥[1] 苏志鹏[1] 吴近森[1] 

机构地区：[1]温州医学院附属第一医院神经外科,325000

出　　处：《中华医学杂志》2012年第39期2739-2742,共4页National Medical Journal of China

基　　金：国家自然科学基金（30800347）

摘　　要：目的探讨腺病毒介导多巴胺2型受体短链亚型基因（D2S）联合溴隐亭药物干预对体外培养的原代人垂体无功能腺瘤（NFPA）细胞凋亡的影响。方法应用重组腺病毒载体pAd-D2S—EGFP将D2S基因导人原代贴壁培养的人NFPA细胞中，免疫荧光染色检测感染后细胞内D2S蛋白表达变化，转染后联合溴隐亭药物干预，并以CCK-8法检测加药后细胞生长活性，并用Hoechst染色细胞核凋亡形态学检测评估转染后药物干预对细胞活性的影响。结果pAd-D2S—EGFP能够成功感染体外贴壁培养的原代人NFPA细胞，转染D2S—EGFP联合溴隐亭药物干预能够抑制NFPA细胞生长，溴隐亭干预后第48小时时，实验组细胞活性为（40±5）％，与单纯加药组（97±5）％及空载体组（90±9）％相比，差异有统计学意义（P〈0．05）。实验组Hoechst染色观察到明显的凋亡现象。结论提高体外培养NFPA细胞的D2S表达会增加肿瘤细胞对溴隐亭治疗的敏感性。Objective To explore the inhibitory effect of non-functioning pituitar adenoma （NFPA） cells after a combined treatment of adenovirus mediated D2S gone and bromocriptine in vitro. Methods Adenovirus containing dopamine 2 receptor short isoform （ D2S ） gene was used to infect NFPA cells. The transfection of D2S gene into NFPA cells was confirmed by immunofluorescence. And cell apoptosis of infected cells treated by bromocriptine was evaluated with CCK-8 assay in vitro. Results When D2S gone transfection and bromocriptine was used in combination, the survival rate of NFPA cells significantly decreased （40 ± 5 ）% versus the control group （97 ±5 ）% and the pAd-EGFP transfection combined bromocriptine treatment group （ 90 ±9 ） % （ P 〈 0. 05 ） . Conclusion The combined treatment of adenovirus-mediated D2S gene and bromocriptine can effectively induce the apoptosis of NFPA cells on primary culture and increase the sensitivity of NFPA to dopamine agonist.

关 键 词：腺病毒科 溴隐亭 催乳素瘤 基因 

分 类 号：R73[医药卫生—肿瘤]