2',4'-二羟基-4',6'-二甲氧基查尔酮在肺腺癌相关细胞中对IL-8表达的影响  

Effect of 2',4'-Dihydroxy-4',6'-Dimethoxychalcone on IL-8 Expression in Lung Adenocarcinoma-associated Cells

作　　者：钱单东[1] 孙睿[1] 吴琼[1] 李引乾[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农业学报》2012年第10期12-16,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：台湾农业生物科学与技术计划(96AS-1.2.1-ST-a1)

摘　　要：为研究2′,4′-二羟基-4′,6′-二甲氧基查尔酮(查尔酮)对肺腺癌组织白细胞介素-8(IL-8)分泌的影响,分别处理在肺腺癌组织细胞中主要存在的早期癌细胞、高侵袭力的癌细胞、肿瘤相关的巨噬细胞,研究分析该化合物对以上3类细胞各自表达IL-8的影响。采用实时定量PCR、ELISA、报告基因分析方法检测IL-8的表达量,利用EMSA分析其调控机制。结果表明,该单体在肺腺癌早期细胞CL1-0中具有抑制肿瘤血管生成因子IL-8产生的能力,其抑制效果达50%。经过EMSA分析,肺腺癌侵染能力强的细胞CL1-5具有增强AP-1、NF-κB因子对DNA调节区的结合能力,从而促进IL-8的表达,其增强表达效果达30%。在肿瘤相关巨噬细胞中,该单体可以有效抑制肿瘤血管新生因子IL-8的表达,其抑制表达效果达24.9%。说明查尔酮在早期肿瘤中具有抑制IL-8表达的作用。There are three major groups in lung adenocarcinoma tissue,including tumor-associated macrophage,high invasion tumor cell and low risk of invasion cell.This study was conducted to investigate the effects of 2′,4′-dihydroxy-4′,6′-dimethoxychalcone（chalcone） on IL-8 expression in lung adenocarcinoma.We focused on the influence of secretion and expression of IL-8 in the preceding three kinds of cells treated with the single compound mentioned above.The methods such as quantitative real-time PCR,ELISA,Dual-Luciferase Reporter Assay System were used to detect the volume of expressed IL-8 and the Electrophoretic Mobility Shift Assay was employed to assay the upstream regulatory mechanism.The series of analysis indicated that the single compound was able to inhibit secretion of IL-8 in CL1-0,with the inhibition effect of 50%.Conversely,both of upstream regulatory factors of IL-8 gene called Active Protein-1（AP-1）,NF-κB which the DNA-binding active were enhanced after treating the single compound in conditioned medium-stimulated high risk of invasion cell line CL1-5,and their enhancing effect was about 30%.The similar phenomenon emerged in tumor-associated macrophages,with the inhibition effect of 24.9%.The result indicated that chalcone has a positive effect on inhibiting IL-8 expression during early-stage of lung adenocarcinama progress.

关 键 词：肺腺癌 白细胞介素-8 血管新生 

分 类 号：R734.2[医药卫生—肿瘤]