耦合二氧化碳减排的微藻产业化培养技术  

CO_2 sequestration coupled with industrial cultivation of microalgae

作　　者：张峰[1,2] 向文洲[1] 萧邶[1] 陈平原 

机构地区：[1]中国科学院南海海洋研究所,广州510301 [2]中国科学院研究生院,北京100049 [3]三亚海王海洋生物科技有限公司,三亚572000

出　　处：《微生物学报》2012年第11期1378-1384,共7页Acta Microbiologica Sinica

基　　金：广东省科技计划项目(2009B080701093);广东海洋渔业推广专项(A201101I05;A201001I04);海洋公益项目(201005031-5);Supported by the Major Project of Chinese National Programs for Fundamental Research and Development(2010CB126500)

摘　　要：【目的】建立耦合CO2减排的微藻培养技术,在减排CO2的同时,有效降低微藻产业化生产的成本。【方法】以两种微藻所具有的pH快速漂移与高碱适应特性为原理,通过CO2防逸罩简易装备的设计和构建,建立二氧化碳减排技术。【结果】将该技术应用于微藻培养,在嗜碱绿球藻MC-1的CO2减排小试培养中,CO2防逸罩的安装使得通入培养物后全部逸出的CO2被碱性藻液完全吸收,在海水钝顶螺旋藻新藻株(HS331)的工厂化中试培养中,其平均产率达9.54g/(m2.d1),碳源成本降低了57%,并有效避免了碳酸根离子导致的海水钙镁离子沉淀的产生;将该技术进一步应用到螺旋藻产业化培养,在保证获得高质量产品的同时,其年产量提高了20%,每年NaHCO3用量减少了66%,碳源成本减少了58%,年减排CO2量约45吨。【结论】本研究实现了CO2减排技术在螺旋藻产业化培养中的应用,有效降低了螺旋藻生产成本,为建立产业化的CO2减排新技术奠定了重要的理论与技术基础。[Objective] In order to reduce CO2 emission and lower the cost of microalgal industrial production at the same time,a technology was developed to combine CO2 sequestration with microalgal cultivation.[Methods] The technology was based on rapid pH drift and high pH adaptability of two microalgal species,Chlorococcum alkaliphilus MC-1 and Spirulina platensis.A simple structure,CO2 leakage prevention covering-box,was designed to collect CO2 escaped from culture medium when CO2 gas was injected into the culture.[Results] In the small-scale outdoor cultivation of MC-1,CO2 escaped from culture was all absorbed by culture with the help of covering-box.Results from the pilot-scale cultivation of S.platensis（HS 331） combined with the CO2 addition technology show that the cost of carbon source was remarkably reduced and deposition of CaCO3 and MgCO3 was effectively avoided.In addition,the average productivity was rather high [9.54g/（m2·d1）].In the large-scale cultivation of S.platensis,the annual yield was increased by 20% and high quality product was obtained with the CO2 addition technology.In addition,66% of NaHCO3 was saved,more than 58% of carbon cost was reduced,and about 45 tons of CO2 was sequestrated.[Conclusion] The technology was successfully applied in industrial production of S.platensis and the cost of producing S.platensis was dramatically reduced.The study would provide new insight into carbon dioxide sequestration.

关 键 词：微藻 钝顶螺旋藻 CO2减排 pH漂移 防逸罩 

分 类 号：X173[环境科学与工程—环境科学]