恒河猴糖尿病动物模型血清超氧化物歧化酶和丙二醛的检测及意义  

An Examination on SOD and MDA in the Serum of Macaques Monkey and Its Clinical Significance

作　　者：朱华[1] 刘颖[1] 秦川[1] 

机构地区：[1]卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,100021

出　　处：《医学研究杂志》2012年第10期24-26,共3页Journal of Medical Research

基　　金：国家科技重大专项基金资助项目(2009ZX09503-025)

摘　　要：目的对糖尿病恒河猴血清中的超氧化物歧化酶(SOD)和丙二醛(MDA)进行动态检测,探讨其在在非人灵长类糖尿病并发肾病模型中的应用价值。方法健康恒河猴5只,小剂量30mg/kg多次静脉注射链脲佐菌素(STZ),血糖稳定后观察血清中SOD和MDA的含量。连续观察13个月。结果造模后动物血清中的SOD值降低,在8个月出现显著性差异(P<0.05),11个月出现极显著性差异(P<0.01)。MDA含量持续上升,9个月出现显著性差异(P<0.05),13个月出现极显著性差异(P<0.01)。血糖与SOD呈负相关(F=-0.186,-0.323,P<0.05),血糖与MDA呈正相关(F=0.128,0.087,P<0.05),SOD与MDA无相关关系。结论测定血清SOD,MDA含量可以在一定程度上反应恒河猴糖尿病微血管病变的程度。Objective To study the content of superoxied dismutase （SOD） and MDA in serum of macaques Monkey and its clinical significance in macaques Monkey. Methods We used streptozotocin （30mg/kg of body weight, intravenously,3days） to five macaques. The dynamic changes of superoxied dismutase （SOD） and MDA in serum were measured for 13 months. Results As a result,the SOD in macaques Monkey＇s serum were lower than those of before injection, and the difference was of statistically significance at the 8th month （ P 〈 0.05）. The level of MDA in the serum were higher than those of before injection, and the difference was of statistically significance at the 9th month（ P 〈 0.05 ）. Significantly negative correlations were observed between SOD and Glu（ F = - 0. 186, - 0. 323, P 〈 O. 05 ）. Significantly positive correlations were observed between MDA and Glu（ F =0. 128, 0. 087 ,P 〈 0.05 ）. There was no correlation between SOD and MDA. Conclusion The results showed that the detection of SOD and MDA reflectes the viability of antioxidase in diabetic ma- caques Monkey. The cause of diabetes is related with free radical.

关 键 词：超氧化物歧化酶 丙二醛 糖尿病 恒河猴 

分 类 号：R743[医药卫生—神经病学与精神病学]