MTT法测定异搏定联合化疗药物对胶质瘤细胞U251化疗敏感性评估  

Effect of Verapamil Combinated with Chemotherapy Drugs on Sensitivity of U251 Glioma Cell Line by the MTT Assay

作　　者：何国龙[1] 金许洪[1] 尹剑[1] 马跃辉[1] 秦福创[1] 叶欣[1] 

机构地区：[1]杭州市红十字会医院神经外科,310003

出　　处：《医学研究杂志》2012年第10期86-88,共3页Journal of Medical Research

基　　金：杭州市科技局资助项目(20061133B12)

摘　　要：目的探讨应用四甲基偶氮唑盐(MTT)检测胶质瘤细胞U251对异搏定联合化疗药物的化疗敏感性。方法利用MTT法检测异搏定分别联合化疗药物(阿霉素、长春新碱、足叶乙甙)在不同作用时间点和不同剂量的作用下胶质瘤细胞U251的存活率。结果异博定联合化疗药物具有一定的协同作用。随着化疗药物作用时间的延长,细胞存活度在各组中均有减少。72h时间点,阿霉素组细胞存活率为34.57%,联合长春新碱组细胞存活率为13.44%,足叶乙甙组细胞存活率为62.71%,组间比较有统计学差异。结论异搏定联合化疗药物与化疗敏感性存在一定关系,为临床化疗选择合适的化疗剂量和化疗周期提供了实验依据。Objective To study the effect of verapamil combinated with chemotherapy drugs on sensitivity of U251 glioma cell line by 3 - （4,5 - dimethylthiahiazol - 2 - yl ） - 2,5 - diphenytetrazolium bromide （MTT） assay. Methods The cell survival rate of the U251 glioma cell line under the effect of different chemotherapeutic agents （doxorubicin, vincristine, etoposide） and at different time points of a series of doses were detected by MTT assay. Results The combination of chemotherapeutic agents and verapamil showed syn- ergistic effect. With the action of the chemotherapeutic drugs, cell survival rate decreased in each group. In the time point of 72 hours, cell viability of doxorubicin group was 35.72% , vincristine group was 16.37% , etoposide group was 61.72% , and there was significant difference between groups. Conclusion The relationship between the doses of chemotherapeutic agents with verapamiland sensitivity of chemotherapy gives a basis for choosing aoorooriate clinical chemotheranv doses.

关 键 词：联合化疗 MTT法 胶质瘤 化疗敏感性 

分 类 号：R734.2[医药卫生—肿瘤]