卫星搭载对羊草种子萌发及细胞学效应的研究  

Effects of seed germination and cytological characteres of Leymus chinensis embarked by satellite

作　　者：潘多锋[1] 张月学[1] 申忠宝[1] 王建丽[1] 张瑞博[1] 李道明[1] 高超[1] 邸桂俐[1] 刘录祥[2] 钟鹏[3] 

机构地区：[1]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [3]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086

出　　处：《草原与草坪》2012年第5期17-21,共5页Grassland and Turf

基　　金：国家科技支撑项目(2008BAD97B07);黑龙江省农业科技创新工程种子创新基金项目(2010-10)资助

摘　　要：试验研究了"实践八号"航天育种卫星搭载羊草种子后的萌发及根尖细胞学效应。结果表明:太空诱变后羊草种子的萌发能力降低,但与对照差异不显著(P>0.05);太空诱变促进了羊草根尖细胞的有丝分裂活动,分裂指数较对照增加24.17%;太空诱变使羊草根尖细胞在有丝分裂过程中产生微核、染色体断片、染色体粘连、落后染色体、游离染色体等畸变类型。其中,单微核是主要的核畸变类型,染色体断片为主要的染色体畸变类型。"实践八号"卫星搭载对羊草根尖细胞有明显的诱变效应。Effects of seed germination and cytological characters of Leymus chinensis embarked by satellite＂Shijian8＂ were studied in this paper.The results showed that space mutagenesis reduced seed germination ability,but not significantly compared with control（P0.05）.Space mutagenesis promoted activity of mitosis and the mitotic index was 24.17% higher than that of control.Meanwhile,chromosome aberrations such as micronucleus,chromosome fragments,chromosome bridge,chromosome conglutination,lagging and so on were observed in root tip cells.Single micronucleus was primary type of nuclear aberration,and chromosome fragments for chromosome aberration.Satellite＂Shijian8＂ had significant effects on root tip cells of Leymus chinensis.

关 键 词：太空诱变 羊草 萌发 有丝分裂指数 染色体畸变 

分 类 号：Q813.1[生物学—生物工程]