^(18)F-FDG ^(18)F-FLT PET显像评价肺腺癌放射治疗疗效的实验研究  

Experimental Research of ^(18)F-FDG and ^(18)F-FLT PET on Therapeutic Response in Radiotherapy for Pulmonary Adenocarcinoma

作　　者：戴东[1] 徐文贵[1] 宋秀宇[1] 朱研佳[1] 朱湘[1] 张莹[1] 

机构地区：[1]天津医科大学附属肿瘤医院分子影像及核医学诊疗科,天津市肿瘤防治重点实验室,天津市300060

出　　处：《中国肿瘤临床》2012年第20期1505-1508,共4页Chinese Journal of Clinical Oncology

基　　金：天津市自然科学基金重点项目(编号:08JCZDJC23700);天津市教委课题项目(编号:20080133);天津市卫生局科技基金项目(编号:2010KZ80)资助~~

摘　　要：目的:评价^(18)F-FDG和^(18)F-FLT PET显像在早期评价肺腺癌放射治疗疗效中的作用。方法:40只A549荷肺腺癌小鼠随机分为对照组、5 Gy组、10 Gy组、15 Gy组和30 Gy组共5组,分别对5个剂量组的荷A549裸鼠模型于放疗前1天和放疗后3天、1周、2周和4周行^(18)F-FDG和^(18)F-FLTPET显像,测量移植瘤大小、SUV值和T/N值。显像后每组随机处死2只裸鼠,行移植瘤免疫组化法检测Ki-67及TK-1表达。分析裸鼠移植瘤放疗前后^(18)F-FDG、^(18)F-FLT PET-CT显像T/N值变化及其与Ki-67表达的相关性。结果:放疗前荷瘤裸鼠^(18)F-FDG、^(18)F-FLT PET-CT显像均可清晰显示移植瘤,据其T/N值(均>1,5)可作出正确诊断。Ki-67、TK-1阳性表达率随放疗时间延长和放疗剂量增加而降低。^(18)F-FDG、^(18)F-FLTPET-CT显像T/N值与反应细胞增殖状况的指标Ki-67表达具有较好的相关性。^(18)F-FLT PET-CT显像T/N值与增殖指标Ki-67及TK-1表达相关程度优于^(18)F-FDG。结论:接受照射后,^(18)F-FLT在肿瘤内的摄取变化可以反映肿瘤细胞的增殖状态,^(18)F-FLT PET-CT显像可以用于肺腺癌放射治疗效果早期评价。Objective： This study aims to evaluate early the efficacy of 18F-FLT and 18SF-FDG PET/CT imaging systems in assessing the tumor response of radiotherapy for pulmonary adenocarcinoma. Methods： A total of 40 A549 xenografts with human pulmonary adenocarcinoma were treated with different dosages （0, 5, 10, 15, and 30 Gy） of X-ray irradiation （radiotherapy）. Changes in tumor volume during the entire experimental period were observed. 18SF-FDG and 18F-FLT PET/CT imaging systems were employed before radiotherapy and at 3 d,week, 2 weeks, and 4 weeks after radiotherapy. SUVmax and T/N were analyzed. Immunohistochemical analysis of the xenografts was conducted to detect the proliferation expression of Ki-67. The T/N values of the18F-FDG and18F-FLT PET/CT imaging systems and the corresponding indexes of Ki-67 before and after radiotherapy were compared. Results： Both imaging systems identified the xenografts before irradiation and distinguished malignancy according to the T/N values （T/N〉1.5） of xenografts. The positive expression rates of Ki-67 decreased as the radiation time was prolonged and as the dosage was increased. The T/N values of the xenografts in the 18F-FDG and 18F-FLT PET/CT images of each time point were correlated with the positive expression rates of Ki-67. The correlation in 18F-FLT PET/CT imaging was better than that in 18F-FDG imaging. Conclusion： Changes in 18F-FLT uptake reflected proliferation. 18F-FLT PET/CT imaging can be used for the early evaluation of radiotherapy for pulmonary adenocarcinoma.

关 键 词：肺腺癌放射治疗 疗效评价脱氧葡萄糖脱氧胸苷正电子发射断层计算机成像 计算机断层显像 

分 类 号：R734.2[医药卫生—肿瘤]