高低分级乳腺癌及导管上皮不典型增生γ-tubulin Nek2 mRNA表达分析及其意义  

Significance ofγ-tubulin and Nek2 mRNA Expressions in High/Low-grade Carcinoma and in Atypical Ductal Hyperplasia

作　　者：牛昀[1,2] 王淑玲[2] 刘铁菊[3] 吕淑华[2] 蒋伶活[1] 

机构地区：[1]天津大学,天津市300072 [2]天津医科大学附属肿瘤医院乳腺病理科 [3]天津医科大学

出　　处：《中国肿瘤临床》2012年第20期1530-1534,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目(编号:30471967;30872519)资助~~

摘　　要：目的：研究不同分级乳腺癌及不典型增生病变中心体γ-tubulin和调控因子Nek2mRNA表达情况及其意义。方法：选取正常乳腺、导管上皮不典型增生、高低级别导管内癌、高低级别浸润性导管癌6组样本，采用原位杂交定性及半定量检测180例γ-tubulin和Nek2mRNA表达情况，采用实时定量RT-PCR测定80例γ-tubulin和Nek2mRNA表达水平，并行统计学分析。结果：各组癌γ-tubulinmRNA、Nek2mRNA表达均明显高于正常组织（P均〈0．01）；各组间差异具有统计学意义（X2=37．519、36．912，P〈0．001）；在不同癌组织之间，γ-tubulin或Nek2表达的两两比较，差异无显著性（P均〉0．05）；在各组中两指标mRNA表达无显著性差异（P均〉0．05）；两指标各自定量和半定量表达总体变化趋势具有一致性；原位杂交分析显示导管上皮不典型增生中γ-tubulin或Nek2mRNA表达与低级别癌比较无统计学差异（X2分别为1．200、0．659、1．148、2．700，P值分别为0．273、0．417、0．284、0．100）；但在定量分析显示导管上皮不典型增生与低级别癌比较有显著差异（P均〈0．01）。结论：γ-tubulin及Nek2mRNA过表达及协同作用，与乳腺上皮细胞的异常增殖、不典型增生向低级别乳腺癌恶变转化可能具有相关性。Objective： To analyze the mRNA expressions of the centrosome Y-tubulin and its regulatory factor Nek2 in high/low-grade carcinoma and in atypical ductal hyperplasia and to evaluate the mechanism of breast cancer evolution. Methods： The breast tissue samples （six groups） were collected from normal tissues, atypical hyperplasia, highand low-grade ductal carcinoma in situ, and high and low-grade of invasive ductal carcinoma. The mRNA expression levels of Y-tubulin and Nek2 were detected by in situ hybridization in 180 samples and by real-time qRT-PCR in 80 samples. Results： The positive rate and quantity ofy-tubulin and Nek2 mRNA expressions in high/low-grade ductal carcinoma in situ and in invasive ductal carcinoma significantly increased compared with those in normal tissues （P〈0.01）. A significant difference was observed among the all six groups （X2=37.519, X2=36.912, P〈0.001）. The difference of mRNA expression levels between any two groups of the carcinoma was not statistically significant （P〉0.05）. The expressions and the abnormal up-tendencies of y-tubulin and Nek2 mRNA were not statistically significant. Comparing the positive rate of expression between the atypical ductal hyperplasia and low-grade carcinoma, the results were X2 = 1.200, 0.659, 1.148 and 2.700, respectively （P〉0.05）, However, the quantitative analysis of these samples showed that statistical difference between them was significant （P〈0.01）. Conclusion： The abnormal mRNA expression of Y-tubulin and Nek2 may have an important function in the successive proliferation of the malignant transformation process in mammary cellula epithelialis.

关 键 词：高低分级乳腺癌 导管上皮不典型增生 Γ-TUBULIN NEK2 MRNA表达 

分 类 号：R737.9[医药卫生—肿瘤]