精制扑炎痛后的回收乙醇在天然药物化学实验中再利用  

Studies on the Recovered Ethanol Used in Refining of Benoral Reuse in Natural Medicinal Chemistry Experiments

作　　者：袁春玲[1] 贾云宏[1] 王亚娟[1] 

机构地区：[1]辽宁医学院药学院,辽宁锦州121001

出　　处：《辽宁医学院学报》2012年第5期401-403,I0003,共4页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：2011年辽宁医学院教改研究项目;编号:2011A07

摘　　要：目的研究将精制扑炎痛后的回收乙醇在天然药物化学实验中再利用。方法减压蒸馏用于精制扑炎痛后的废乙醇,称取大黄粗粉100 g,用300 mL乙醇热回流提取(使回收乙醇占95%新鲜乙醇的比例为0∶1、1∶5、1∶3、1∶2、1∶1),提取分离得到的蒽醌苷元进行薄层层析鉴定。结果用各种比例乙醇提取的提取物Ⅰ分别展开的斑点与标准品大黄酸所对应的斑点Rf值相同,提取物Ⅱ分别展开的斑点与标准品大黄素所对应的斑点Rf值相同,提取物Ⅲ展开分别有两个斑点,一个与标准品大黄酚所对应的斑点Rf值相同,另一个与标准品大黄素甲醚所对应的斑点Rf值相同,初步确定提取物Ⅰ中有大黄酸成分,提取物Ⅱ中有大黄素成分,提取物Ⅲ中有大黄酚和大黄素甲醚成分,且不存在其他杂质斑点。结论回收得到的用于精制扑炎痛后的废乙醇可以用于大黄中蒽醌类成分的提取。Objective To study the reuse of recovered Ethanol that has been used in refining of Benoral in natural medicinal chemistry experiments.Methods Vacuum distillation of waste ethanol was used in the refining of Benoral.100 g crude rhubarb powder was weighted,300 mL ethanol was extracted by heat reflux,and TLC was used to identify the extracted and separated Anthraquinone glycosides.Results Rf values between the spot evolved respectively by extractionⅠextracted by various proportion ethanol and the spot of standard reagent rhein were the same,Rf values between the spot evolved respectively by extraction Ⅱ extracted by various proportion ethanol and the spot of standard reagent emodin were the same;evolved extraction Ⅲ had two spots respectively,Rf values between one spot and the spot of standard reagent chrysophanol were the same.The other spot＇s Rf value and the spot of standard reagent physcion＇s Rf value were the same,which preliminarily confirmed that there was rhein in the extraction,emodin in the extraction Ⅱ,chrysophanol and physcion in the extraction Ⅲ,and there was no impurity spot.Conclusion The recovery of waste ethanol used in the refining of Benoral can be used in extraction of Anthraquinone glycosides.

关 键 词：实验废液 乙醇 回收 再利用 

分 类 号：TQ461[化学工程—制药化工]