RP-HPLC法测定不同产地余甘子药材和鞣质有效部位中3种成分的含量  

Determination of gallic acid, corilagin and ellagic acid in Fructus Phyllanthi and tannins effective fraction with RP-HPLC

作　　者：张鸿雁[1] 沙磊[1] 石任兵[1] 张兰珍[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中华中医药杂志》2012年第11期2834-2838,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助(No.39970929);北京中医药大学自主选题资助(No.2009TYB22-JS027);北京中医药大学创新团队(No.2011-CXTD-12)~~

摘　　要：目的:建立反相-高效液相色谱法(RP-HPLC)同时测定余甘子药材及鞣质有效部位中没食子酸、柯里拉京和鞣花酸含量。方法:采用Diamonsil C18柱(250mm×4.6mm,5μm),以甲醇-0.2%冰醋酸水溶液为流动相,梯度洗脱;检测波长为270mm和254nm;流速为1mL/min;柱温为30℃。对线性关系、精密度、稳定性、重复性、回收率各方法学进行考察。结果:没食子酸、柯里拉京和鞣花酸线性范围分别为0.1728-1.0368μg、0.0717-0.1792μg、0.2440-0.4880μg;平均回收率(n=6)分别为100.60%、100.65%、102.16%,RSD分别为2.83%、2.45%、1.65%。结论:建立的方法简便,重现性好,可用于余甘子药材和鞣质有效部位中没食子酸、柯里拉京和鞣花酸的含量测定。Objective： To establish an RP-HPLC method for the assaying of the content of gallic acid,corilagin and ellagic acid in Fructus Phyllanthi and tannins constituent.Methods： The determination was carried out with a Diamonsil C18 column（250 mm×4.6 mm,5 μm） and column temperature was 30℃.The mobile phase was the system of methanol 0.2% aqueous acetic acid with flow rate of 1 mL/min,the UV detection was set at 270nm and 254nm.Results： The linear range of 0.1728-1.0368 μg for gallic acid,0.0717-0.1792 μg for corilagin and 0.2440-0.4880 μg for ellagic acid.The average recoveries（n=6） of gallic acid,corilagin and ellagic acid were 100.60%（RSD=2.83%）,100.65%（RSD=2.45%）,102.16%（RSD=1.65%）,respectively.Conclusion： The established method is simple and reproducible,and can be used for determination of gallic acid,corilagin and ellagic acid in Fructus Phyllanthi and tannins effective fraction.

关 键 词：余甘子 鞣质有效部位 没食子酸 柯里拉京 鞣花酸 高效液相色谱 

分 类 号：R29[医药卫生—民族医学]