荧光分析法测定肉类食品中诺氟沙星的残留  

Determination of Norfloxacin Residue in Meat Product by Fluorescence Spectrometry

作　　者：蔡其洪[1,2] 许桂芬[1] 阮丽琴[1] 李春来[1] 

机构地区：[1]莆田学院医学院,福建莆田351100 [2]食品安全分析与检测教育部重点实验室(福州大学),福建福州350108

出　　处：《沈阳大学学报（自然科学版）》2012年第5期19-22,共4页Journal of Shenyang University：Natural Science

基　　金：福建省教育厅科技计划项目(JA09197);食品安全分析与检测教育部重点实验室(福州大学)开放基金(FS10001);福建省科技计划重点项目(2011Y0048)

摘　　要：采用Al 3+增敏诺氟沙星的荧光强度,建立荧光分析法测定肉类食品中诺氟沙星的残留.优化了分析实验体系的最佳pH值、Al 3+质量浓度和反应时间,利用荧光法测定,诺氟沙星的线性方程为F=15.89+36.50C,线性范围在0.5～250ng.mL-1之间,方法检出限为0.032ng.mL-1,回收率在81.00%～99.31%之间.该方法简单、快速、灵敏、准确,可用于肉类食品中诺氟沙星含量的测定.A spectrofluorimetric method for the determination of norfloxacin residue in meat product was described,based on Al3＋ can strengthen the relative fluorescence intensity of norfloxacin evidently.Under the optimum conditions of pH,Al3＋ concentration and reaction time,norfloxacin was detected by fluorescence spectrometry.The linear equation for norfloxacin was F=15.89＋36.50C,the linear range of the determination was 0.5 ng·mL-1 to 250 ng·mL-1,the detection limit was 0.032 ng·mL-1,and the recoveries were in the range of 81.00%~99.31%.This method is easy,swift,sensitive and accuracy.It can be applied to the determination of norfloxacin in meat product directly.

关 键 词：诺氟沙星 荧光分析法 肉类 含量测定 

分 类 号：O657.3[理学—分析化学]