锂-匹罗卡品致癫痫大鼠模型时程机制探讨  

Explore of lithium pilocarpine induced epilepsy rat model of scheduling mechanism

作　　者：金戈[1] 王俊平[1] 王春红[1] 严丹丹[1] 姜殿君[1] 郝伟[1] 付首用[1] 周桦地[1] 

机构地区：[1]沈阳医学院基础医学教研室,110034

出　　处：《中国现代医药杂志》2012年第10期9-11,共3页Modern Medicine Journal of China

基　　金：辽宁省教育厅项目(编号:L2010547);沈阳医学院青年基金(编号:20092017)

摘　　要：目的观察锂-匹罗卡品致癫痫大鼠模型各时程c-fos mRNA和fos蛋白表达水平。方法采用免疫组化和实时荧光定量PCR技术检测fos蛋白和基因水平的表达。结果 fos基因和蛋白在癫痫持续状态后1h升高,6h同时达高峰。24h表达仍有升高。结论癫痫持续状态后6hfos蛋白和mRNA表达同时达高峰。Objective To observe the lithium pilocarpine induced epilepsy rat model each time c-fos mRNA and los protein expression levels. Methods Using immunohistochemistry and real-time quantitative PCR to check fos protein and gene level expression. Results Fos gene and protein also increased 1 hour after the seizures, reached a peak at the same time of 6 hours. The expression of 24 hours was still elevated. Conclusion Six hours after status epilepticus protein and mRNA expression reached a peak at the same time.

关 键 词：癫痫模型 c—fos 实时荧光定量 PCR 

分 类 号：R742.1[医药卫生—神经病学与精神病学]