CO_2气腹对结直肠癌细胞侵袭转移能力的影响  

Influence of CO_2 pneumoperitoneum on invasion capacity of colorectal cancer cells

作　　者：梁鸿[1] 张辉[1] 张超[1] 王旺河[1] 曹俊[2] 黄克俭[2] 江弢[2] 裘正军[2] 

机构地区：[1]河南省人民医院腹腔镜微创外科,郑州市450003 [2]上海交通大学附属第一人民医院普外科,上海市200080

出　　处：《中华实用诊断与治疗杂志》2012年第11期1089-1091,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的探讨CO2气腹对结直肠癌细胞侵袭转移能力的影响。方法 99例结直肠癌患者分为腹腔镜手术组50例和开腹手术组49例,通过免疫组织化学方法检测2组患者结直肠癌手术组织标本中缺氧诱导因子-1α、β-链蛋白和E-钙黏蛋白的表达,并进行比较。结果腹腔镜组与开腹组患者手术标本中缺氧诱导因子-1α(72%与67%)、β-链蛋白(38%与47%)和E-钙黏蛋白(50%与49%)的表达率比较差异无统计学意义(P>0.05);腹腔镜组与开腹手术组β-链蛋白(68%与65%)和E-钙黏蛋白(66%与57%)在细胞膜中表达缺失差异无统计学意义(P>0.05)。结论 CO2气腹不影响缺氧诱导因子-1α的表达及β-链蛋白和E-钙黏蛋白的表达及结合,可能不促进结直肠癌的侵袭转移。Objective To investigate the influence of CO2 pneumoperitoneum on invasion and metastasis capacity of colorectal cancer cells. Methods A total of 99 cases of coloreetal cancer were divided into laparoscopy group （n=50） and open abdomincal operation group （n=49）. The expressions of hypoxia inducible factor-1 alpha （HIF-1α）, β-catenin and E-cadherin were detected with immunohistochemistry in two groups. Results There was no significant difference in the expressions of HIF-la （72% vs 67%）, β-catenin （38% vs 47%） and E-cadherin （50% vs 49%）, and in the loss expressions of β-catenin protein （68% vs 65%） and E-cadherin protein （66% vs 57%） between laparoscopy group and open abdomincal operation group （P〉0.05）. Conclusion CO2 pneumoperitoneum may not influence the expression of HIF-la, as well as the expression and binding of β-catenin and E-cadherin, and may not promote the invasion and metastasis of colorectal cancer.

关 键 词：结直肠癌 CO2气腹 缺氧诱导因子-1α Β-链蛋白 E-钙黏蛋白 

分 类 号：R735.3[医药卫生—肿瘤]