频域光学相干断层扫描对激光诱导的大鼠脉络膜新生血管的定量测量  

Quantitative measurement of laser-induced choroidal neovascularization with spectral-domain optical coherent tomography

作　　者：王雯秋[1] 汪枫桦[1] 王泓[1] 宋正宇[1] 吴颖[1] 孙晓东[1] 

机构地区：[1]上海交通大学附属第一人民医院眼科,200080

出　　处：《中华实验眼科杂志》2012年第11期1009-1012,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：国家“973计划”课题项目（2011CB707506）;国家自然科学基金面上项目（30973259）;国家自然科学基金青年基金项目（81100678）

摘　　要：背景脉络膜新生血管（CNV）是导致眼部多种视力严重损伤疾病的病理改变之一。频域光学相干断层扫描（SD-OCT）检查可实现在眼部组织的连续观察、测量和分析。目的采用SD-OCT对激光诱导的大鼠CNV进行量化检测，评估SD-OCT对CNV进行活体定量检测的可行性及应用价值。方法摘取30只BN大鼠的左眼，采用532nm激光进行视网膜光凝的方法建立CNV模型，激光功率为200mw，光斑直径为100μm，曝光时间为50ms，以光凝后产生气泡或伴有轻度出血为击破Brunch膜和造模成功的标志。分别于光凝后3、7、14、21d采用荧光索眼底血管造影（FFA）检查法判断造模成功与否并进行评分，同时采用SD-OCT对CNV形成过程进行活体实时测量，CNV膜厚度（CMT）为视网膜内界膜至视网膜色素上皮（RPE）-脉络膜血管层之间的最大距离，结合光凝后14d的组织病理学检查结果对CNV测量结果进行评估和验证。BN大鼠的右眼作为对照眼。结果FFA检查结果表明，光凝后7d开始出现视网膜的盘状荧光素渗漏，14d渗漏区扩大。光凝后7、14、21d，CNV的渗漏评分分别为（1．6±0．4）、（2．5±0．6）、（2．4±0．5）分，3个时间点渗漏评分的比较差异有统计学意义（F=13．11，P〈0．01）；光凝后14d、21d，CNV渗漏评分明显高于光凝后7d，差异均有统计学意义（P〈0．05）。光凝后7、14、21d，SD-OCT测量的cMT分别为（76．33±10．09）、（102．03±14．21）、（98．03±13．76）μm，3个时间点CMT的差异有统计学意义（F：23．25，P〈0．01）；光凝后14d和21d，CMT与7d相比明显增加，差异均有统计学意义（P〈0．05）。SD-OCT检查结果与FFA结果相比，随着时间的延长CNV的发展趋势具有一致性。光凝后14d，组织病理学检查证实CNV形成，进一步证实上述改变。结论532nm激光能成功诱导BN大鼠形成CNV，具有模型稳定、成模率高的优点。Background The neovascular form of the disease usually causes severe vision loss in a number of eye diseases. Special-domain optical coherence tomography（SD-OCT） provides high-quality in retinal imaging and the possibility of the measurement in vivo. Objective This study aimed to investigate the feasibility of SD-OCT dynamically measuring choroidal neovascularization （CNV）. Methods CNV was induced in 30 left eyes of 30 clean Brown Norway （BN）rats by retinal photocoagulation with the laser parameter as follows： wavelength 532 nm, exciting power 200 roW,spot diameter 100 Ixm and irradiating time 50 ms. Bubble or less retinal bleeding was thought as Brunch membrane breakage and CNV model establishment. Fundus fluorescein angiography（FFA） was performed to determine the establishment of CNV model and scored based on the fluorescein leakage on 3,7,14,21 days after photocoagulation. Meanwhile,CNV memberane thickness （CMT） was dynamically measured in vivo as the maxiume value from retinal inner limiting membrane through choroidal vessel layer in various time points. Histopathologic examination was used in the 14th day to evaluate and verify the result of SD-OCT. The right eyes were as controls. Results FFA examination showed that disc-like leakage of fluorescein appeared in 7 days and extended in 14 days after photocoagulation with the scores of 1.6±0. 4,2.±0.6 and 2. 4±0.5 in 7,14 and 21 days, showing a significant difference among them（F= 13. 11 ,P〈0.01 ）, The fluorescein leakage score was significantly higher in 14 and 21 days than that of 7 days（ both P〈0.05）. CMT measured by SD-OCT was（76.33±10. 09） , （ 102. 03±14.21 ） and（98. 03±13.76）μm in 7,14 and 21 days after photocoagulation respectively,with a significant difference among 3 time points （F= 23.25, P〈0.01 ）, and that in 14 and 21 days was significantly declined in comparison with 7 days （both P〈0.05）. The results of SD-OCT showed a consistent tendency with that of FFA. Histopathologieal examinatio

关 键 词：脉络膜新生血管 频域光学相干断层扫描 动物模型 生物测量 

分 类 号：R773.4[医药卫生—眼科]