瘦素对大鼠气道平滑肌细胞凋亡的影响  

The apoptosis effect of leptin on rat′s airway smooth muscle cells

作　　者：倪文静[1] 朱述阳[1] 刘文静[1] 吴瑕[1] 陈云峰[1] 赵玲[1] 

机构地区：[1]徐州医学院附属医院呼吸科,江苏徐州221002

出　　处：《重庆医学》2012年第31期3295-3297,F0004,共4页Chongqing medicine

摘　　要：目的探讨瘦素对大鼠气道平滑肌细胞(ASMCs)凋亡的影响。方法体外培养大鼠ASMCs。Western blot法测定ASMCs上瘦素受体的表达。不同浓度(0、20、40、60、80、100μg/mL)的瘦素及100μg/mL瘦素+BY-0961Rleptin receptor(L)干预培养的大鼠ASMCs 48h后,Annexin V-FITC/PC测定细胞凋亡率,Western blot法测定半胱氨酸天冬氨酸蛋白酶(caspase-3)和B细胞淋巴瘤/白血病-xl(Bcl-xl)蛋白表达。结果 ASMCs上有瘦素受体的表达;细胞的凋亡率与瘦素浓度呈负相关;瘦素对ASMCs抗凋亡作用与浓度呈正相关,caspase-3表达减少,Bcl-xl表达增加。结论大鼠ASMCs表面有瘦素受体的表达。瘦素可以抑制体外培养的大鼠ASMCs的凋亡,且这种作用呈浓度依赖性。Objective To study the apoptosis effect of leptin on rat＇s airway smooth cells（ASMCs）. Methods Rat ASMCs were derived from rat airway tissue and then cultured in vitro. ASMCs were stimulated with the different concentrations of leptin（0,20, 40,60,80,100 μg/mL）, 100 μg/mL of leptin and BY-0961Rleptin receptor（L） for 48 h. After that,the rates of cell apoptosis were detected by flow cytometry, respectively. The protein expression of caspase-3 and Bcl-xl were measured by Western blot respective- ly. Results There was the expression of leptin receptor on ASMCs;There was negative correlation between cell apoptosis and dif- ferent concentrations of leptin;Leptin could inhibit the apoptosis of ASMCs at 48 h with different concentrations and inhibit the ex- press of caspase-3, and increase the express of Bcl-xl. These anti-apoptosis effects of leptin had positive correlation with the concen- trations of it. Conclusion There were leptin receptors on the rats ASMCs. Leptin can inhibit the apoptosis of ASMCs in vitro through the combination with leptin receptors.

关 键 词：瘦素 肌细胞 平滑肌 气管 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 Bcl-X蛋白质 大鼠 

分 类 号：R363[医药卫生—病理学]