重症急性胰腺炎大鼠肝损伤HMGB 1/TLR4mRNA的表达  

Changes of HMGBl and Toll-like receptor gene expression of livers in acute liver injury complicated with severe acute pancreatitis rats

作　　者：黄鹏[1] 吴河水[2] 王春友[2] 

机构地区：[1]广西中医药大学附属瑞康医院肝胆外科,南宁530011 [2]华中科技大学同济医学院附属协和医院胰腺外科,武汉430022

出　　处：《疑难病杂志》2012年第11期854-856,共3页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助项目(No.30200272)

摘　　要：目的研究重症急性胰腺炎(SAP)大鼠肝损伤中高迁移率族蛋白B1(HMGB1)和Toll样受体(TLR)4mRNA的表达。方法采用十二指肠闭襻法制作大鼠SAP模型。50只动物分为假手术组(S组)、胰腺炎组(P组)。P组于建模后6、12、24、48 h分批剖杀,S组于术后6 h剖杀。观察血清淀粉酶、CRP、ALT和AST及肝组织IL-6和TNF-α的变化,RT-PCR方法检测各组不同时点肝组织HMGBl mRNA和TLR4 mRNA的表达。结果与S组比较,P组血清淀粉酶、CRP、ALT、AST、肝组织IL-6和TNF-α浓度升高(P<0.05)。与S组比较,P组大鼠6 h肝组织TLR4mRNA表达开始增高,术后12 h肝组织TLR4 mRNA表达迅速达到峰值(P<0.05);同时,P组HMGB1 mRNA于12 h快速上升,24～48 h时一直保持上升趋势(P<0.05);结论SAP大鼠肝组织内HMGB1和TLR4的基因表达上调;其表达增高可能在SAP肝损伤的发生、发展中起重要作用。Objective To investigate the changes of HMGB1 and Toll-like receptor 4 gene expression of livers in a- cute liver injury complicated with severe acute pancreatitis （SAP） rats. Methods Fifty SD male rats were randomly divided into Sham-operated group （ n = 10） and P group （ n =40）. Levels of amylase, ALT, AST, CRP, IL-6 and TNF-α were observed. HMGB1 mRNA and TLR4 mRNA expression in the livers were measured by RT-PCR. Results Liver injuries were aggravated, the levels of serum amylase, CRP, ALT, AST, IL-6 and TNF-α were increased in livers （ P 〈 0.05）. TLR4 mR- NA could be detected in livers with low values in sham-operated group, but they were significantly increased at 6 hours in SAP group, peaking at 12 hours （ P 〈 0.05 ）. HMGB1 mRNA were significantly increased at 12 hours in SAP group, peaking at 48 hours （ P ~ 0.05 ）. Conclusion These data suggested that expression of HMGB1 mRNA and TLR4 mRNA were increased in livers in SAP, which may play an important role in mediating pro-inflammatory cytokine synthesis and release. Up-regulation of HMGB1 mRNA and TLR4 mRNA expression in livers may be involved in the genesis and the development of acute liver injury complicated in SAP.

关 键 词：胰腺炎 重症 急性 高迁移率族蛋白B1 TOLL样受体 肝损伤 大鼠 

分 类 号：R576[医药卫生—消化系统]