Determination of metronidazole in a rat stomach by HPLC for obtaining basic data of the eradication therapy of Helicobacter pylori  

作　　者：Mai Kubodera Tadakazu Tokumura Yoshiharu Machida 

机构地区：[1]Pharmaceutical Research Laboratories,Nihon Generic Co.Ltd.,Kannonndai 1-25-4,Tsukuba,Ibaraki 305-0856,Japan [2]Department of Pharmaceutical Sciences,School of Pharmacy,International University of Health and Welfare,Kitakanemaru 2600-1,Ohtawara,Tochigi 324-8501,Japan [3]Department of Drug Delivery Research,Hoshi University,Ebara 2-4-41,Shinagawa,Tokyo 142-8501,Japan

出　　处：《Journal of Pharmaceutical Analysis》2012年第5期378-381,共4页药物分析学报（英文版）

摘　　要：In the eradication therapy of Helicobacter pylori changes of antibiotics as these concentrations or amount in the stomach after oral administration were not clear. A simple and accurate method for determining the concentration of metronidazole （MTZ） in homogenate of rat stomach was developed in order to obtain basic data to design a pharmaceutical preparation having targeting ability to the surface of gastric-mucosa. This method included a deproteinization process by methanol, separation with reversed-phase high-performance liquid chromatography, and detection with an ultraviolet wavelength of 370 nm. Regression analysis showed that the method was linear over a standard curve nmge from 5 ug/mL to 2000 g/mL. The inter-day precision and accuracy values between thc ranges were 5.0% or better and -7.5 to 5.2%, respectively. The newly developed method was applied to an analysis of gastric samples after oral administration of MTZ at a dose of 5 mg/kg. It was found that the residual MTZ in the stomach was determined within 5 h after dosing. This method is useful for monitoring MTZ in stomach after its oral administration to rats.In the eradication therapy of Helicobacter pylori changes of antibiotics as these concentrations or amount in the stomach after oral administration were not clear. A simple and accurate method for determining the concentration of metronidazole （MTZ） in homogenate of rat stomach was developed in order to obtain basic data to design a pharmaceutical preparation having targeting ability to the surface of gastric-mucosa. This method included a deproteinization process by methanol, separation with reversed-phase high-performance liquid chromatography, and detection with an ultraviolet wavelength of 370 nm. Regression analysis showed that the method was linear over a standard curve nmge from 5 ug/mL to 2000 g/mL. The inter-day precision and accuracy values between thc ranges were 5.0% or better and -7.5 to 5.2%, respectively. The newly developed method was applied to an analysis of gastric samples after oral administration of MTZ at a dose of 5 mg/kg. It was found that the residual MTZ in the stomach was determined within 5 h after dosing. This method is useful for monitoring MTZ in stomach after its oral administration to rats.

关 键 词：METRONIDAZOLE HPLC HOMOGENATE STOMACH RAT 

分 类 号：R927[医药卫生—药学]