脑缺血肺损伤大鼠肺组织中脑源性神经营养因子的表达变化  

Expression Changes of BDNF in Lung of Rats Subjected to Brain Ischemia

作　　者：但齐琴[1] 戴萍[1] 王盛兰[2,3] 李劲涛[4] 张云辉[2,3] 

机构地区：[1]四川大学华西医院转化神经科学研究中心,成都610041 [2]昆明理工大学附属昆华医院 [3]云南省第一人民医院呼吸内科,昆明650032 [4]昆明医科大学神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2012年第6期897-900,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2009CD199,No.2010ZC206,No.2011FZ272,No.2012fb086)资助

摘　　要：目的研究脑缺血肺损伤大鼠肺组织中脑源性神经营养因子(BDNF)基因的表达变化,探讨BDNF的生物学作用。方法成年SD大鼠46只,随机分为假手术组和脑缺血肺损伤组,每组23只,其中5只用于神经行为评价和肺水肿测定,用HE染色观察肺病理学炎症反应;每组8只动物用于RT-PCR检测肺组织BDNFmRNA含量变化;5只用ELISA检测肺组织BDNF蛋白含量变化;5只动物取肺组织进行免疫组化染色观察BDNF在肺的分布。结果脑缺血后3d大鼠神经功能明显受损,肺组织明显充血,水肿,大量炎性细胞浸润。BDNF mRNA表达较假手术组高(P<0.05),BDNF蛋白水平亦高于假手术组(P<0.05)。BDNF免疫阳性产物定位于肺上皮和平滑肌细胞。结论脑缺血大鼠有明显肺水肿,肺组织BDNF表达上调,提示BDNF与脑缺血肺水肿有关。Objective To investigate the expression of brain-derived neurotrophic factor （BDNF） in lung injury induced by brain ischemia in rats. Methods 46 adult SD rats were assigned randomly to sham operation group and brain ischemia lung injury group （BILI,n= 23 in each group）. Rats were subjected brain ischemia and allowed to survived 3 d. After performed neurological functional severe deficit evaluation, lung edema was observed （n= 5）. The BDNF expression for its mRNA and protein in lung tissues was determined by using ELISA （n= 5） and RT-PCR technique （n= 8）. The localization of BDNF was also determined by immunohistochemistry （n = 5）. Results After brain ischemia for 3 days, the severe neurological functional deficit and edema in lung were seen. BDNF was located in cytoplasma of smooth muscle and epithial cells in the lung. The level of BDNF mRNA （indicated by RT-PCR） and the protein level （indicated by ELISA） were all upregulated at 3 days after brain ischemia （P〈0. 05）. Conclusion Lung edema occurred after brain iscbemia in rats is concomitant with BDNF expression, which consists of the mechanism involved lung injury induced by brain ischemia.

关 键 词：大鼠 脑缺血肺损伤 脑源性神经营养因子 

分 类 号：R743[医药卫生—神经病学与精神病学]