脑缺血肺损伤大鼠肺组织trkB的表达变化  

Expression of trkB Gene in the Pulmonary Tissue of Rats with Lung Injury Induced by Cerebral Ischemia

作　　者：赵珊[1,2] 荣荣[3] 但齐琴[4] 张云辉[1,2] 

机构地区：[1]昆明理工大学附属昆华医院 [2]云南省第一人民医院呼吸内科,昆明650032 [3]安徽省立医院老年医学科,合肥230001 [4]四川大学华西医院转化神经科学中心,成都610041

出　　处：《四川大学学报（医学版）》2012年第6期901-903,958,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2010ZC206,No.2012fb086)资助

摘　　要：目的观察脑缺血肺损伤大鼠肺组织酪氨酸蛋白激酶B(trkB)的表达变化,并探讨其与肺损伤的关系。方法成年SD大鼠分为假手术组和脑缺血肺损伤组,每组13只动物。术后3d处死大鼠,每组中5只取肺组织进行免疫组化染色、8只取肺组织进行RT-PCR和Western blot,检测trkB蛋白在肺组织的分布、trkB mRNA和蛋白的表达变化。结果 trkB分布于气管上皮和平滑肌。与假手术组相比,trkB mRNA和蛋白在损伤肺组织表达增加(P<0.05)。结论脑缺血肺损伤大鼠肺组织trkB表达水平明显上调,提示其与脑缺血肺损伤有关。Objective To investigate the expression of tyrosine kinase B （trkB） in lung tissue of rats with lung injury induced by brain ischemia. Methods Twenty six adult SD rats were divided into sham group and brain ischemia lung injury （BILI） group. All rats were sacrificed at 3 days after the operation of modeling, lung tissues were then harvested to measure the protein and mRNA level of trkB by the methods of western blot and RT-PCR, the location of trkB positive cells was observed by immunochemistry study. Results trkB mRNA level in the lung tissue of rats with brain ischemia presented a significant increase, in corresponding to the upregulation of BDNF protein levels, when compared with sham one （P〈0. 05）. trkB was localized in endothelia cells and smooth muscle. Conclusion The upregulated expression of trkB expression may be associated with lung injury after brain ischemia.

关 键 词：大鼠 脑缺血肺损伤 酪氨酸蛋白激酶B 

分 类 号：R743[医药卫生—神经病学与精神病学]