脑缺血大鼠肺组织中IL-6的表达变化  

IL-6 Expression in Lung of Rats Subjected to Brain Ischemia

作　　者：谭燕[1,2] 蒋淑一[1,2] 但齐琴[3] 夏宜兰[1,2] 李劲涛[4] 张云辉[1,2] 

机构地区：[1]昆明理工大学附属昆华医院 [2]云南省第一人民医院呼吸内科,昆明650032 [3]四川大学华西医院转化神经科学中心,成都610041 [4]昆明医科大学神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2012年第6期907-909,917,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2010ZC206,No.2011FZ272)资助

摘　　要：目的探讨脑缺血肺组织白细胞介素-6(IL-6)的表达变化。方法将成年SD大鼠随机分为假手术组和脑缺血肺损伤组,用线栓法建立大脑中动脉堵塞脑缺血肺损伤模型。脑缺血后24h取肺组织用RT-PCR,48h用Western blot技术检测肺组织IL-6的表达量。脑缺血后72h用免疫组织化学技术检测IL-6蛋白定位分布。结果肺组织IL-6基因和蛋白表达在脑缺血肺组织明显增加,与对照组比较,差异有统计学意义(P<0.05)。IL-6阳性染色主要位于气道上皮细胞胞和巨噬样细胞。结论脑缺血后肺组织IL-6表达明显上调,提示IL-6与脑缺血肺损伤有一定关系。Objective To study the expression changes of interleukin-6 （IL-6） in the lung of rats with brain ischemia. Methods Adult SD rats were divided into sham operation group and brain ischemia lung injury （BILI） group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. RT-PCR was applied to detect the IL-6 expression level of lung at 24 h after brain ischemia. The IL-6 protein in lung at 48 h after brain ischemia was detected with Western blot. Immunohistochemistry method was used to demonstrate the IL-6 protein location in lung at 72 h after brain ischemia. Results Increased immunostaining, protein level and mRNA expression for IL-6 were found in lung at investigated time point after brain ischemia. Conclusion IL-6, as a crucial preinflammatory factor, could be related to airway inflammation in rats induced by brain ischemia.

关 键 词：脑缺血肺损伤 白细胞介素-6 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]