脑缺血大鼠肺组织中IL-1β的表达变化  

Expression Changes of IL-1β in Lung of Rats Subjected to Brain Ischemia

作　　者：岳倩宇[1] 但齐琴[2] 荣荣[3] 陈隽[1] 李劲涛[4] 张云辉[1] 

机构地区：[1]昆明理工大学附属昆华医院·云南省第一人民医院呼吸内科,昆明650032 [2]四川大学华西医院转化神经科学中心,成都610041 [3]安徽省立医院老年医学科,合肥230001 [4]昆明医科大学神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2012年第6期910-913,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2010ZC206,No.2011FZ272)资助

摘　　要：目的了解脑缺血肺损伤后肺组织中白细胞介素-1β(IL-1β)表达的变化,探讨其功能意义。方法成年SD大鼠随机分为假手术组和脑缺血肺损伤组,采用线栓法建立大脑中动脉脑缺血肺损伤模型。于术后24h用RT-PCR(n=8),48h用蛋白免疫印迹技术(n=8)检测肺组织IL-1β表达变化;术后72h用免疫组织化学技术检测肺组织内IL-1β的定位分布(n=5)。结果肺组织IL-1β基因和蛋白表达在脑缺血后24h及48h明显增加,与对照组比较,差异有统计学意义(P<0.05)。IL-1β主要分布在气道上皮细胞、巨噬细胞及中性粒细胞。结论脑缺血肺损伤组织IL-1β表达明显上调,提示IL-1β可能在脑缺血肺损伤炎症反应中发挥作用。Objective To study the expression changes of interleukin 1β （IL-1β） in the lung of rats with brain ischemia. Methods Adult SD rats were divided into sham operation group and brain ischemia lung injury （BILI） group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. Lungs were harvested from rats at different time point respectively. RT-PCR （24 h）, Western blot （48 h） and immunohistochemistry （72 h） were employed to detect the expressional changes and the distributions of 1L- 1β in the lung tissues. Results IL-1β immunohistochemical positive reaction was observed in epithelia cell and neutrophil as well as macrophage. Increased protein level and mRNA expression for IL-1β were found in lung after brain ischemia compared with those of sham group. Conclusion IL-1β, as a crucial inflammatory factor, could be associated with airway inflammation in lung following brain ischemia in rats.

关 键 词：脑缺血肺损伤 白细胞介素-1Β 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]