脑缺血肺损伤大鼠肺组织TNF-α的表达变化  

Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia

作　　者：廖凤[1] 但齐琴[2] 杜润芬[1] 李劲涛[3] 张云辉[1] 

机构地区：[1]昆明理工大学附属昆华医院·云南省第一人民医院呼吸内科,昆明650032 [2]四川大学华西医院转化神经科学中心,成都610041 [3]昆明医科大学神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2012年第6期914-917,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2010ZC206,No.2011FZ272)资助

摘　　要：目的探讨脑缺血后损伤肺组织肿瘤坏死因子-α(TNF-α)的表达变化。方法成年SD大鼠随机分为假手术组、脑缺血肺损伤组(n=13),其中每组5只用于免疫组化检测TNF-α在肺的定位分布,余8只分别用于RT-PCR和Western blot检测TNF-αmRNA和蛋白水平变化。术后24h取肺组织行RT-PCR、术后48h取肺组织行Western blot检测肺组织TNF-αmRNA和蛋白表达水平;术后3d取肺组织行免疫组织化学染色检测肺组织内TNF-α的定位分布。结果肺组织TNF-αmRNA和蛋白表达在脑缺血肺组织明显增加,与假手术组比较,差异有统计学意义(P<0.05)。TNF-α主要分布在气道上皮细胞和巨噬样细胞。结论脑缺血后损伤肺组织TNF-α表达上调,提示其可能与脑缺血肺损伤炎症反应有关。Objective To investigate the expression of tumor necrosis factor-α （TNF-α） in the lung tissue of rats with lung injury induced by brain ischemia. Methods The rat model of lung injury induced by brain ischemia was established. At 24 h, 48 h, 72 h after brain ischemia, lung tissues were harvested from each rats, the expressions of TNF-α mRNA and protein and its distributions in the lung tissue were measured by the methods of RT-PCR （n=8）, Western blot （n=8）, and immunohistochemistry （n=5）, respectively. Results The increase of TNF-α mRNA and protein levels were found in the lung tissues after brain ischemia, when compared with sham group （P〈0.05）. The morphological study with immunohistochemical staining observed TNF-α positive reaction in epithelial cells and some macrophages in the lung tissues after brain ischemia. Conclusion The expression of TNF-α in the lung tissue could be upregulated in the rats with brain ischemia.

关 键 词：脑缺血肺损伤 肿瘤坏死因子-Α 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]