BDNF对脑缺血肺损伤大鼠肺组织IL-1β表达的影响  

Effects of BDNF Antibody to Block IL-1β Expression and Lung Injury in Rats Subjected to Brain Ischemia

作　　者：荣荣[1] 杨婷婷[2] 李劲涛[3] 但齐琴[4] 张云辉[2] 

机构地区：[1]安徽省立医院老年医学科,合肥230001 [2]昆明理工大学附属昆华医院·云南省第一人民医院呼吸内科,昆明650032 [3]昆明医科大学神经科学研究所,昆明650031 [4]四川大学华西医院转化神经科学中心,成都610041

出　　处：《四川大学学报（医学版）》2012年第6期918-921,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2010ZC206)资助

摘　　要：目的探讨脑源性神经营养因子(BDNF)对脑缺血肺损伤肺水肿的影响及对白细胞介素-1β(IL-1β)表达的调节。方法将SD大鼠随机分为假手术组、脑缺血肺损伤组、脑缺血肺损伤给予BDNF抗体处理组(BDNF抗体干预组),每组13只。于BDNF抗体干预后3d取大鼠肺组织样本,HE染色(n=5)观察各组肺水肿及中性粒细胞浸润;免疫组化染色(n=5)和Western blot技术(n=8)检测脑缺血后肺组织IL-1β的定位与相对表达量。结果气道上皮细胞和中性粒细胞可见IL-1β表达。BDNF抗体处理后3d,肺水肿及中性粒细胞浸润减轻;肺组织IL-1β表达明显减少,与脑缺血肺损伤组比较,差异有统计学意义(P<0.05)。结论 BDNF抗体处理可减轻脑缺血肺损伤大鼠肺组织水肿及降低IL-6的表达。Objective To explore the effects of brain-derived neurotrophic factor （BDNF） antibody to block lung injury and interleukin-1β （IL-1β） expression in the rats with brain ischemia. Methods Thirty nine SD rats were divided into sham group, brain ischemia lung injury （BILI） group, and BDNF antibody treated group. Inflammatory lung injury was induced by brain ischemia in the later two groups, and BDNF antibody was given through intraperitoneal injection to the rats for 3 days in BDNF antibody group. Lung tissue samples were harvested from the rats in each group at the 3^rd day after brain ischemia. Lung edema degree was evaluated by HE staining. The protein expressions of IL-1β in the lung tissues were measured by the methods of immunohistochemistry （n= 5） and Western blot （n=8）. Results The increased immunostaining of IL-1β were found in the lung tissue at 3 days after brain ischemia, which indicated the upregulated expression of IL-1β protein in the lung injury induced by cerebral ischemia. The block of BDNF antibody resulted in a significant decrease of IL-1β expression, as well as the decrease of lung edema. Conclusion BDNF, as a crucial factor, could regulate airway inflammation injury in brain ischemia rats via activating IL-1β expression.

关 键 词：脑缺血肺损伤 白细胞介素-1Β 大鼠 脑源性神经营养因子 

分 类 号：R743[医药卫生—神经病学与精神病学]