PIM-1基因沉默对前列腺癌细胞生长抑制作用的研究  

Effect of Silence of PIM-1 Expression on Proliferation of Prostate Cancer Cell Line

作　　者：张晓光[1] 徐勇[2] 张志宏[2] 杨阔[2] 

机构地区：[1]天津市第三中心医院泌尿外科,300170 [2]天津市泌尿外科研究所

出　　处：《天津医药》2012年第11期1089-1091,I0003,共4页Tianjin Medical Journal

基　　金：天津市科技支撑计划项目(项目编号:07ZCGYSF01000)

摘　　要：目的:探讨RNA干扰(RNAi)沉默PIM-1基因表达对前列腺癌细胞体外生长的影响。方法:将RNAi重组质粒(PPIM1-shRNA-3)经脂质体介导转染前列腺癌PC-3细胞,用G418筛选稳定转染、PIM-1基因沉默的PC-3细胞。用空质粒载体(pRNAT-U6.1/Neo)稳定转染的PC-3细胞和正常培养的PC-3细胞作为对照,进行体外研究。利用细胞计数法分别检测3组PC-3细胞的增殖能力。利用流式细胞技术分别检测3组PC-3细胞的细胞周期及凋亡情况。结果:与两对照组PC-3细胞相比,PIM-1基因沉默组的PC-3细胞在培养48、72和96h的细胞计数显著减少(P<0.01),细胞周期中G1期细胞的比例显著升高,而S期细胞的比例显著降低(P<0.01),发生早期凋亡的细胞比例明显升高(P<0.01)。结论:PIM-1基因沉默可以使得PC-3细胞的细胞周期进程受阻,抑制细胞增殖,并诱发细胞凋亡。PIM-1可以作为前列腺癌基因治疗的有效靶点,具有潜在的临床应用价值。Objective： To investigate the effect of RNAi-mediated silencing of PIM-1 on the growth of prostate cancer cells. Methods： The recombinant plasmid vectors of RNAi （P^PIM-shRNA-3）were transfeeted into PC-3 cell line by liposome. The stable transfection cells,which PIM-1 gene silenced,were screened by G418. The PC-3 cells, which were stably transfected with empty plasmid vectors （pRNAT-U6.1/Neo）, and wild PC-3 cells were obtained as contrast. Cell-counting assay and flow cytometry（FCM）were used separately to determine the proliferation activity, cell cycle and apoptosis of PC- 3 cells in three groups. Results： Compared with control group, the count of the PIM-1 gene silenced PC-3 cells euhured at 48 h,72 h and 96 h decreased significantly （P 〈 0.01）. The percentage of the cells in GI stage increased while cells in S stage decreased significantly （P 〈 0.01）. At the same time, much more apoptotic cells were observed in the PIM-1 gene silenced PC-3 cell line （P 〈 0.01）. Conclusion： The silence of PIM-1 gene can result in the block of the cell cycle, suppression of cell proliferation and increasing apoptosis in PC-3 cells. PIM-1 may serve as a effective target of gene therapy for prostate cancer in clinical practice.

关 键 词：前列腺肿瘤 RNA干扰 原癌基因蛋白质c—pim-1基因 肿瘤抑制 肿瘤抑制蛋白质类 基因沉默 

分 类 号：R737.25[医药卫生—肿瘤]