HPLC多波长切换法同时测定野生山楂中芦丁、金丝桃苷及槲皮素含量  

Simultaneous Determination of Rutin,Hyperoside,Quercetin in Wild Fructus Crataegi by Multiple Wavelength RP-HPLC

作　　者：张雪莹[1] 张杨 张建逵[3] 韩荣春[3] 康廷国[3] 王冰[3] 

机构地区：[1]沈阳佰佳安医疗健康用品有限公司,辽宁沈阳110031 [2]大连华立金港药业有限公司,辽宁大连116100 [3]辽宁中医药大学,辽宁大连116600

出　　处：《中华中医药学刊》2012年第11期2418-2420,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技基础条件平台项目子课题(2005DKA21000)

摘　　要：目的:建立同时测定山楂果实中芦丁、金丝桃苷及槲皮素的分析方法。方法:采用HPLC多波长切换法,色谱柱为Agilent Eclipse XDB-C18柱(4.6mm×150mm,5μm):流动相:乙腈(A)-0.025mol/L磷酸水(B),梯度洗脱依次为0～15min(18%A～18%A),15～30min(18%A～40%A);检测波长363nm(0～18min),371nm(1～30min);流速:0.9mL/min;柱温:28℃。结果:芦丁、金丝桃苷及槲皮素的线性范围分别为0.06296～0.3148μg、1.0512～5.2560μg、0.06240～0.3120μg。平均加样回收率分别为100.59%、99.27%、100.03%,RSD分别为2.00%、1.99%、1.92%。结论:该方法简便、快速、准确,重现性好,可用于山楂果实中芦丁、金丝桃苷及槲皮素的同时测定。Objective：To establish a method for simultaneous determination of rutin, hyperoside, quercetin in Fructus Crataegi. Methods ： A multiple wavelength HPLC method was developed. The analysis was performed on an Agilent Eclip- seXDB- C18 column（4.6mm ×150mm,5μm）. A mixture of acetonitrile（A） -0. 025mol/L phosphoric acid（B） with gradient elution as the mobile phase was adopted. The gradient elution program was 0 - 15min（ 18% A - 18% A）, 15 30min（18%A-40%A）. The wavelength was monitored 363nm（0 - 18rain）,371nm（ 18 -30min）. The flow rate was 0.9mL/min. The column temperature was 28℃. Results：The linear response ranges of rutin, hyperoside and quercetin were 0. 06296 - 0. 3148μg, 1. 0512 -5. 2560μg, 0. 06240 - 0. 3120μg respectively. The average recoveries were 100. 59% ,99.27%, 100.03% with RSD of 2.00%, 1.99%, 1.92% respectively. Conclusion：The method is simple and reproducible for determining rutin, hyperoside, quercetin in Folium Crataegi.

关 键 词：反相高效液相色谱 山楂 芦丁 金丝桃苷 槲皮素 

分 类 号：R284.1[医药卫生—中药学]