丹参酮ⅡA磺酸钠对兔急性心梗再灌注后无复流的影响及与氧化应激的关系  

Beneficial Effects and the Potential Oxidative Stress Mechanism of Tanshinone ⅡA on the No-reflow Phenomenon in Rabbits after Acute Myocardial Infarction and Reperfusion

作　　者：王琳莉[1] 黄抒伟[1] 窦丽萍[1] 车庆[1] 陆明[1] 王寅[1] 殷舟[1] 林冬铭[2] 

机构地区：[1]浙江中医药大学附属第二医院,浙江杭州310005 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2012年第11期2513-2516,I0009,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2009CA038)

摘　　要：目的:评价丹参酮ⅡA磺酸钠预处理对兔急性心肌梗死(AMI)再灌注后无复流的防治作用,探讨是否与抑制氧化应激相关。方法:新西兰大白兔40只随机分成4组,即AMI对照组(I/R)、丹参酮再灌注治疗组(DS-201)、丹参酮冠脉结扎前治疗组(DS-201-pre)、假手术组(sham),每组各10只。各组AMI前、后,再灌注后,测定血清中肌钙蛋白I(cTNI)和超氧化物歧化酶(SOD)的含量,最终进行病理学分析,评估缺血范围(LA/LVA)及无复流范围(NRA/LA)。结果:(1)AMI后90min时,SOD及cTNI水平在I/R组、DS-201组和DS-201-pre三组间差异无统计学意义(均P>0.05);再灌注120min时DS-201-pre组及DS-201组的血清cTNI水平低于I/R组(均P<0.01),SOD水平高于I/R组(P<0.01,P<0.05);DS-201-pre组血清cTNI水平低于DS-201组(P<0.05),DS-201-pre组与DS-201组SOD水平差异无显著性(P>0.05)。(2)各组病理染色所测的冠脉结扎区心肌范围(LA)基本一致(均P>0.05)。DS-201组及DS-201-pre组无复流范围均小于I/R组(均P<0.05),DS-201-pre组与DS-201组间差异无显著性(P>0.05)。结论:机体氧化应激损伤可能是急性心梗再灌注后无复流发生的重要机制,丹参酮ⅡA磺酸钠可能通过提高机体抗氧化能力,抑制氧化应激,从而达到保护心肌,有效防治无再流现象的发生。Objective ： To evaluate the effects of Tanshinone ⅡA pre - treatment on myocardial oxidative stress and myo- cardial no - reflow in a rabbit model of acute myocardial infarction and reperfusion. Methods ： Forty rabbits were divided randomly into 4 groups （ n = 10 ） ： I/R （ischemia/reperfusion） group, DS - 201 group （ treatment with DS - 201 before reperfusion）, DS -201 -pre group（ treatment with DS -201 before AMI）, sham operation group. Serum ccTNI levels and SOD content were detected by colorimetric method. Finally, pathological analysis was done. Thioflavine S, Evans blue and Triphenyhetra zolium chloride （TTC） staining were performed to evaluate the area of no - reflow, infracted area and risk ar- ea of the heart. Results ： （ 1 ） Serum contents of cTNI and SOD at 60min of AMI had no significant differences among I/R group ,DS- 201 group and DS- 201 -pre group（ both P 〉 0.05 ）;compared with VR group, serum content of cTNI was decreased （ P 〈 0.05 ） , serum content of SOD was increased（ P 〈 0.01, P 〈 0.05 ） in DS - 201 group and DS - 201 - pre group at 120min of reperfusion ;serum content of cTNI was significantly lower while serum contents of SOD had no signifi- cant differences between DS- 201 group and DS- 201 -pre group（ P 〉 0.05 ）. （2）The ligation area（LA） measured by pathological evaluation had no significant difference in all groups（ all P 〉 0.05 ）. The area of no -refiow in the DS -201 group and DS - 201 - pre group were both significantly lower than that in I/R group（ both P 〈 0.05 ） ; the area of no - re- flow in the DS- 201 group and DS- 201 -pre group were similar. Conclusion： Impairment of myocardial oxidative stress in vivo might play an important role in the pathogenesis of no - reflow after acute myocardial infarction and reperfusion. Tanshinone IIA can effectively protect the myocardium, decrease no - reflow area during acute myocardial infarction and reperfusion in rabbits, the mechanism may be related with the e

关 键 词：急性心肌梗死 再灌注 无复流 丹参酮ⅡA磺酸钠 氧化应激 

分 类 号：R-322[医药卫生]