冬春季温室切段扦插快繁马铃薯脱毒苗  

Rapid Propagation of Potato Virus-free Plantlets by Cutting Cuttage in Greenhouse in Winter and Spring

作　　者：高彦萍[1,2] 杨谋[1,2] 李掌[1,2] 柳永强[1,2] 齐恩芳[1] 张荣[1] 张武[1,2] 

机构地区：[1]甘肃省农业科学院马铃薯研究所,兰州730070 [2]甘肃一航薯业科技发展有限责任公司

出　　处：《长江蔬菜》2012年第20期18-20,共3页Journal of Changjiang Vegetables

基　　金：甘肃省农业生物技术研究与应用开发项目(2008-11);陇原青年创新人才扶持计划项目(2008);甘肃省农业科学院农业科技创新专项(2009GAAS18)

摘　　要：在近几年试验研究的基础上，提出冬春季温室切段扦插快繁马铃薯脱毒苗模式。试验结果表明，温室温度25～28℃，相对湿度90％，光照5000～10000lx，脱毒苗切段长2．5～3cm，蘸生根液NAA20mg/L＋KT2mg/L＋GA30．5m∥L，扦插密度1000。1300株，m。，扦插后喷雾生长调节液（NAA5mg/L＋6-BA0．5mg/L＋GA31mg/L）和1／2MS营养液或1，2K5营养液＋植物叶面肥，20—35d后，苗高8～10cm、茎节6—7个时，进行继代切段繁殖，如此重复继代切段繁殖3-4次，脱毒苗增殖20～25倍。On the basis of the previous experiment results in recent years, the article studied the rapid propagation system of virus-free potato plantlets by cutting euttage in greenhouse in winter and spring. The results showed that the appropriate conditions for cutting cuttage and rapid propagation of potato virus-free plantlets were as follows： the temperature was 25- 28℃, with relative humidity of 90%, light intensity of 5000-10 000lx in greenhouse, cuttings length of 2.5-3 era, density of 1 000-1 300 euttings/m2, and the rooting medium was NAA 20 Ing/L＋KT 2 mg/L ＋GA3 0.5 mg/L. The cutting seedlings, sprayed with the growth regulator solution （NAA 5 mg/L＋6-BA 0.5 mg/L＋GA3 1 rag/L） and 1/2 MS nutrient solution or 1/2 K5 nutrient solution＋foliage fertilizer （Huashuo 828）, took roots and germinated very well. After 20-35 days of growth, the seedlings with height of 8-10 cm and 6-7 nodes were used as basic seedlings for the following subculture cutting euttage and breeding. Finally, the virus-free plantlets proliferated 20-25 times through 3-4 subculture cycles of cutting cuttage propagation.

关 键 词：马铃薯 切段扦插 快繁 脱毒苗 

分 类 号：S532[农业科学—作物学]