钙阻断剂伊拉地平在帕金森病大鼠模型中的神经保护作用  

Neuroprotective Effects of Calcium Blocker Isradipine in Rat Model of Parkinson's Disease

作　　者：胡明[1] 陈国华[1] 刘昌勤[2] 

机构地区：[1]武汉市第一医院神经内科,武汉430022 [2]华中科技大学同济医学院附属协和医院神经内科,武汉430022

出　　处：《华中科技大学学报（医学版）》2012年第5期581-584,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的利用6-羟基多巴胺(6-OHDA)损毁的大鼠帕金森病(PD)模型,研究钙阻断剂伊拉地平对神经元的保护作用。方法大鼠给予伊拉地平1周后,纹状体内注射6-OHDA造模,然后继续给药2周。用圆柱体试验和旋转试验评价损毁程度,用酪氨酸羟化酶(TH)免疫组化染色计数多巴胺神经元数量。结果行为学测试表明,伊拉地平预给药大鼠运动功能障碍有所改善。免疫组化染色显示,用药组黑质多巴胺能神经元较模型组显著增多,提示伊拉地平预给药具有神经保护作用。另1组动物在损毁后给药则没有明显改善。结论伊拉地平有希望成为具有神经保护作用的新型抗PD药物。Objective To explore the neuroprotective effects of calcium blocker isradipine in a 6-OHDA lesioned rat model of Parkinson＇s disease（PD）.Methods Rats were pretreated with isradipine for one week before they received a 6-OHDA injection into the striatum,then they were treated with isradipine for two more weeks.Cylinder test and apomorphine-induced rotation test were performed for evaluating the severity of lesion.Immunohistochemistry staining of tyrosine hydroxylase（TH）was used for evaluating the survival of dopaminergic neurons.Results Behavior tests showed that locomotion impairment was improved by pretreatment of isradipine.TH staining indicated elevated number of DA neurons in the substantia nigra.However,rats treated with isradipine after lesion did not show any protective effects.Conclusion Pretreatment with isradipine before lesion protected 6-OHDA-induced neurotoxicity,indicating a novel strategy for the therapy of PD.

关 键 词：钙阻断剂 伊拉地平 帕金森病 神经保护 6-羟基多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]