大豆异黄酮对A549细胞化疗协同作用  

Synergistic antitumor effect of soybean isoflavones combined with chemotherathy on A549 cell

作　　者：尹学哲[1] 金延华[1] 许惠仙[1] 

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《中国公共卫生》2012年第11期1465-1467,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30360113);吉林省科技发展计划(200705426)

摘　　要：目的探讨大豆异黄酮(ISF)联合长春瑞滨(NVB)化疗对A549肺癌细胞增殖影响。方法采用噻唑兰法观察ISF和NVB联用对A549肺癌细胞增殖影响,采用Weeb系数法判定联合用药相互作用,同时采用透射电镜检测A549细胞形态学变化。结果 ISF和NVB单独使用均可时间和浓度依赖性地抑制肺癌A549细胞增殖;20～80 mg/L ISF作用48 h,A549细胞增殖抑制率为15%～23%;8～32 mg/L NVB作用48 h,A549细胞增殖抑制率为53%～80%;ISF浓度在40 mg/L时,ISF和NVB联用后抗A549细胞增殖作用增强,对肺癌细胞生长抑制作用呈量效关系;且NVB浓度在8～32 mg/L时,抑制率为71%～93%,二者有相加或协同作用。结论 ISF和NVB联用可增强对肺癌A549细胞增殖抑制,二者具有协同作用。Objective To assess the inhibitory effect and mechanism of soybean isoflavones（ISF） combined with vinorelbine（NVB） on proliferation of A549 lung carcinoma cells.Methods（3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） assay was used to examine the effects of ISF and NVB on proliferation of A549 cells,and their combined effect was analyzed by Weeb＇s method.Transmission electron microscopic technique was used for the determination of morphology of A549 cell apoptosis.Results ISF and NVB inhibited the proliferation of lung carcinoma cells in a time-and concentration-dependent manner.The inhibitory indexes of ISF at the concentration of 20-80 mg/L were 15%-23%,and the inhibitory indexes of NVB at the concentration of 8-32 mg/L were 53%-80% at 48 hours.The concentration-dependent pattern in the combination of ISF and NVB were also detected with 40 mg/L ISF.The synergistic or additive effect on inhibition of A549 cell proliferation was detected for NVB at the concentration from 8 to 32 mg/L with the inhibitory indexes of 71%-93%.Conclusion ISF combined with NVB can enhance the growth inhibition of A549 lung carcinoma cells and play a synergistic antitumor effect.

关 键 词：大豆异黄酮(ISF) 长春瑞滨(NVB) 肺癌 增殖 形态学 

分 类 号：R155.53[医药卫生—营养与食品卫生学]