癫痫患者CYP2D6＊10基因多态性分析  

The analysis of CYP2D6 10 gene polymorphisms in patients with epilepsy

作　　者：荆洪英[1] 董丽华[1] 谷继卜[1] 侯大平[1] 曲秀梅[1] 张伟智[1] 雷力力[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2012年第5期3-4,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省卫生厅科研项目;编号:2011-389

摘　　要：目的:通过比较本地区健康人与癫痫患者CYP2D6*10基因多态性的差异,探索CYP2D6*10基因多态性与癫痫易感的关系。方法:应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测健康人和癫痫患者CYP2D6*10的基因型分布及等位基因频率,通过χ2检验来比较健康人和癫痫患者的基因型分布差异和等位基因频率差异。结果:健康人和癫痫患者的基因型分布及等位基因频率差异有显著性和高度显著性(P<0.05和P<0.01)。结论:癫痫病的发生与CYP2D6*10的碱基变异具有相关性。Objective：To explore the relationship between CYP2D6＊ 10 gene polymorphisms and the Epilep- sy pathogenesis, by comparing the differences of CYP2D6＊ 10 Gene polymorphisms in healthy populations and patients with epilepsy in the region. Methods：Polymerase chain reaction--restriction fragment length polymorphism （PCR--RFLP） method was applied io detect the genotype distribution and the allele frequencies of CYP2D6＊ 10 in healthy populations and patients with epilepsy, and to compare their differences between them according to Chi--square criterion. Results：There exist significant differences in the genotype distribution and allele frequencies of CYP2D6 ＊ 10 in healthy populations and patients with epilepsy （P 〈 0. 05 ,P 〈 0.01）. Con- clusion：The epilepsy pathogenesis is related with the base variations of CYP2D6＊ 10.

关 键 词：CYP2D6＊10 癫痫 基因多态性 PCR-RFLP 

分 类 号：R742.1[医药卫生—神经病学与精神病学] Q754[医药卫生—临床医学]