癌胚抗原相关细胞黏附分子-6在人肝门部胆管癌细胞株QBC939中的表达及其在侵袭转移中的作用  

Expression of carcinoembryonic antigen-related cell adhesion molecule 6 in human hilar cholangio- carcinoma cell line QBC939 and its role in invasion and metastasis

作　　者：黄尧[1] 曾永毅[1] 刘景丰[1] 林科灿[1] 罗顺峰[1] 张翔[1] 

机构地区：[1]福建医科大学附属第一医院肝病中心肝外科福建省肝病科学研究中心,福州350005

出　　处：《中华实验外科杂志》2012年第11期2117-2119,共3页Chinese Journal of Experimental Surgery

基　　金：福建省自然科学基金资助项目（2011J01162）;福建省卫生厅青年科研课题（2009-2-15）;福建省属高校科研项目基金资助项目（JK2011021）

摘　　要：目的观察癌胚抗原相关细胞黏附分子-6（CEACAM6）在人肝门部胆管癌细胞株QBC939中的表达，探讨其在侵袭转移中的作用。方法实时定量聚合酶链反应（Real-timePCR）检On．0CEACAM6在QBC939细胞中的表达；构建CEACAM6的RNA干扰（RNAi）慢病毒载体，并感染QBC939细胞，Real-timePCR验证RNAi效果；Transwell侵袭试验检测QBC939细胞体外侵袭力的改变。结果CEACAM6在QBC939细胞中高表达。成功构建CEACAM6基因RNAi慢病毒载体并转染QBC939细胞。和对照组比较，RNAi组CEACAM6mRNA表达抑制率为93．1％（P〈0．05）；Tran．swell实验提示RNAi后，侵袭细胞OD570RNAi组（0．09±0．01）明显少于对照组（0．13±0．02），侵袭抑制率为69．2％（P〈0．05）。结论CEACAM6在QBC939细胞中高表达，其表达程度与肝门部胆管癌细胞侵袭转移能力呈正相关。Objective To explore the expression of carcinoembryonic antigen-related cell adhesion molecule 6 （CEACAM6） in human hilar cholangiocarcinoma cell line QBC939 and its roles in invasion and metastasis. Methods Real-time polymerase chain reaction （real-time PCR） was used to detect the ex- pression of CEACAM6 in QBC939 cells. Lentiviral-mediated CEACAM6 small interfering RNA （ siRNA ） was established to infect QBC939 cells. The silencing effect was assayed by real-time PCR. The invasive activity of infected QBC939 cells was analyzed by Transwell invasion assay. Results There was high ex- pression of CEACAM6 in QBC939 cells. Recombinant lentiviral vector expressing siRNA targeting CEACAM6 gone was established successfully and confirmed by PCR and DNA sequencing. The expression of CEACAM6 mRNA in QBC939 cells infected with LV-CEACAM6-siRNA was decreased by 93.1% as compared with control group （P 〈 0. 05 ）. After CEACAM6 gene was knocked down, Transwell test re- vealed that the invasion potency of QBC939 cells was significantly suppressed by 69.2% as compared with control group （ P 〈 0. 05 ）. Conclusion There was high expression of CEACAM6 in QBC939 cells. There is a positive correlation between the expression level of CEACAM6 and the invasion potency of hilar cholan- giocarcinoma.

关 键 词：RNA干扰 癌胚抗原相关细胞黏附分子-6 肝门部胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]